Difference between revisions of "DMV Valproate 100mg/kg Controls"
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| − | Fill in some saline controls for Valproate enhancement of saccharin-LiCl CTA. | + | Fill in of some saline controls for Valproate enhancement of saccharin-LiCl CTA. |
{{Infobox experiment|align=right | {{Infobox experiment|align=right | ||
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| protocol = 202200000006 | | protocol = 202200000006 | ||
| funding = Chair's Fund | | funding = Chair's Fund | ||
| − | | investigators = Mukherjee | + | | investigators = Mukherjee, Houpt |
| species = rat | | species = rat | ||
| date = 2022-12-1 | | date = 2022-12-1 | ||
| type = CTA | | type = CTA | ||
| − | | drugs = | + | | drugs = NaCl, lithium |
| artifacts = | | artifacts = | ||
| data = https://houptlab.org/bartab/expt/?id=DMV | | data = https://houptlab.org/bartab/expt/?id=DMV | ||
| Line 23: | Line 23: | ||
==People== | ==People== | ||
| − | |||
Durba Mukherjee (in absentia) | Durba Mukherjee (in absentia) | ||
| Line 33: | Line 32: | ||
'''Rats arrive (2202-11-22)''' | '''Rats arrive (2202-11-22)''' | ||
| − | Sprague Dawley rats (dob | + | Sprague Dawley rats (dob females: 2022-9-22; males: 2022-9-26 ) |
| − | '''Day 0: Water removed 2022-12- | + | '''Day 0: Water removed 2022-12-10''' |
<br> | <br> | ||
| − | '''Days 1- | + | '''Days 1-10: Water Restriction 2022-12-11 thru 2022-12-20''' |
Weigh rats daily. Water for 10 min, ad lib food. | Weigh rats daily. Water for 10 min, ad lib food. | ||
| Line 48: | Line 47: | ||
<br> | <br> | ||
| − | '''Day | + | '''Day 11: Conditioning Day 2022-12-21''' |
* -15 min: 8 ml/kg of 0.15M LiCl | * -15 min: 8 ml/kg of 0.15M LiCl | ||
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Groups: | Groups: | ||
| − | * DMV01-04 NaCl- | + | * DMV01-04 NaCl-NaCl-Female |
| − | * DMV05-08 NaCl- | + | * DMV05-08 NaCl-LiCl-Female |
| − | * DMV09-12 NaCl- | + | * DMV09-12 NaCl-NaCl-Male |
| − | * DMV13-16 NaCl- | + | * DMV13-16 NaCl-LiCl-Male |
| Line 65: | Line 64: | ||
<br> | <br> | ||
| − | '''Days | + | '''Days 12-23: Preference Tests: 2022-12-22 to 2023-1-4''' |
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 12 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days. | Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 12 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days. | ||
Revision as of 12:28, 21 December 2022
Fill in of some saline controls for Valproate enhancement of saccharin-LiCl CTA.
| DMV Valproate Controls | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMV |
| Start Date | 2022-12-1 |
| Investigators | Mukherjee, Houpt |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | NaCl, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMV |
Data
People
Durba Mukherjee (in absentia)
Agenda
Rats arrive (2202-11-22)
Sprague Dawley rats (dob females: 2022-9-22; males: 2022-9-26 )
Day 0: Water removed 2022-12-10
Days 1-10: Water Restriction 2022-12-11 thru 2022-12-20
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
Day 11: Conditioning Day 2022-12-21
- -15 min: 8 ml/kg of 0.15M LiCl
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg ip of 0.15M LiCl or NaCl
Groups:
- DMV01-04 NaCl-NaCl-Female
- DMV05-08 NaCl-LiCl-Female
- DMV09-12 NaCl-NaCl-Male
- DMV13-16 NaCl-LiCl-Male
ad lib water returned after final injection overnight.
Days 12-23: Preference Tests: 2022-12-22 to 2023-1-4
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 12 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.