Oligo Tail Labeling

From MagnetoWiki
Jump to: navigation, search

For labeling DNA oligonucleotide probes for use for In Situ Hybridization.

Reagents:

Terminal Transferase Labeling Kit,

dATP-alphaS35, (250 uCi vial = 20 µl) Perkin Elmer #NEG034H250UC $172

made first Tuesday of every month

ProbeQuant G-50 micro spin columns, 50 pack, GE Healthcare, # 28-9034-08 $199

Procedure:

Take radioactivity out to melt (1 h if isotope in thick glass vial).

Turn on heat block to 100° C.

[ format as for cDNA Probe Labeling]