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JC & JD Systemic Sodium Selenate

2012-06-15T18:07:17Z

Brown:

Test c-Fos induction

2012-6- Weigh rats JC1-12; JD 1-12

2012- 6- perfuse JC 1-12; JD 1-12

Reuse same rats, shuffled into new groups:

At -2 h, inject with sodium selenate in NaCl 0.15 M, 1 ml/kg at 5 doses:

0mg/kg Na2SeO4
0.5 mg/kg NaSeO4
1 mg/kg NaSeO4
2 mg/kg NaSeO4

and 0.15M LiCl 76 mg/kg as a positive control.

At 0 h, perfuse rats.
Process for c-Fos (~1 week in the lab).

[[Category: CTA Experiments]]

JC & JD Systemic Sodium Selenate

2012-06-15T17:58:51Z

Brown: Created page with 'Test c-Fos induction 2012-6- Weigh rats JC1-5 2012- 6- perfuse JC 1-5 Reuse same rats, shuffled into new groups: At -2 h, inject with sodium selenate in NaCl 0.15 M, 1 m…'

Test c-Fos induction

2012-6- Weigh rats JC1-5

2012- 6- perfuse JC 1-5

Reuse same rats, shuffled into new groups:

At -2 h, inject with sodium selenate in NaCl 0.15 M, 1 ml/kg at 5 doses:

0mg/kg Na2SeO4
0.5 mg/kg NaSeO4
1 mg/kg NaSeO4
2 mg/kg NaSeO4

and 0.15M LiCl 76 mg/kg as a positive control.

At 0 h, perfuse rats.
Process for c-Fos (~1 week in the lab).

JB Systemic Sodium Selenate

2012-06-15T17:58:19Z

Brown: search database broken, so tried adding link to try to create new page

(Brief background of the experiment).

[[Selenium|Sodium Selenate]] (Na2Se04) activates PP2A.

==Agenda==

'''1. Test Conditioned Taste Aversion (23 days)'''

Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min). ''Start Restriction on Tuesday, 2011-11-29''

Weigh rats ''Wednesday, 2011-12-7''

Conditioning day ''Thursday, 2011-12-8'':

4 groups (n=6 per group) : NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl

at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)
:For isotonic solution of sodium selenate: Na2Se04 at 12 mg/ml (63 mM), NaCl at 3.2 mg/ml (55mM) = 300 mOsm

at 0 h, 10 min access to 0.125% saccharin (CS)

at 0.5 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)

at 1.5 h, Return water overnight

Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days. ''End on Friday, 2011-12-23.''

Results: While NaCl-LiCl rats acquired a moderate aversion, the dose of sodium selenate was too high: all of the selenate treated rats acquired a strong CTA, with zero preference for saccharin that persisted for all 14 days of 2-bottle testing.

Conclusion: next time, we'll reduce dose of sodium selenate.

'''2. Test c-Fos induction'''

2012-1-10 perfused JB 1-5

2012-3-14 perfuse JB 6-10

Reuse same rats, shuffled into new groups:

at -2 h, Inject with sodium selenate in NaCl 0.15 M , 1 ml/kg at 4 doses:

*0 mg/kg Na2Se04
*0.5 mg/kg Na2Se04
*1 mg/kg Na2Se04
*2 mg/kg Na2Se04

and 0.15M LiCl 12 ml/kg as a positive control.

at 0 h, perfuse rats.

process for c-Fos (~ 1 week in the lab)

[[JC & JD Systemic Sodium Selenate]]

[[Category: CTA Experiments]]

JB Systemic Sodium Selenate

2011-11-29T18:00:26Z

Brown:

(Brief background of the experiment).

[[Selenium|Sodium Selenate]] (Na2Se04) activates PP2A.

==Agenda==

'''1. Test Conditioned Taste Aversion (23 days)'''

Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min). ''Start Restriction on Tuesday, 2011-11-29''

Weigh rats ''Wednesday, 2011-12-7''

Conditioning day ''Thursday, 2011-12-8'':

4 groups (n=6 per group) : NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl

at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)

at 0 h, 10 min access to 0.125% saccharin (CS)

at 0.5 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)

at 1.5 h, Return water overnight

Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days. ''End on Friday, 2011-12-23.''

'''2. Test c-Fos induction'''

reuse same rats, shuffled into 4 groups: NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl

at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)

at 0 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)

at 1 h, perfuse rats

process for c-Fos (~ 1 week in the lab)

[[Category: CTA Experiments]]

JB Systemic Sodium Selenate

2011-11-29T17:47:33Z

Brown: added category

JB Systemic Sodium Selenate

2011-11-29T17:45:52Z

Brown: added dates

JB Systemic Sodium Selenate

2011-11-29T17:35:58Z

Brown: Created agenda

(Brief background of the experiment).

[[Selenium|Sodium Selenate]] (Na2Se04) activates PP2A.

==Agenda==

'''1. Test Conditioned Taste Aversion (23 days)'''

Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min)

Conditioning day:

4 groups (n=6 per group) : NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl

at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)

at 0 h, 10 min access to 0.125% saccharin (CS)

at 0.5 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)

at 1.5 h, Return water overnight

Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days.

'''2. Test c-Fos induction'''

reuse same rats, shuffled into 4 groups: NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl

at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)

at 0 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)

at 1 h, perfuse rats

process for c-Fos (~ 1 week in the lab)