https://wiki.houptlab.org/api.php?action=feedcontributions&feedformat=atom&user=Jcote 2026-05-23T07:12:20Z User contributions MediaWiki 1.35.8 https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3224 2024-07-24T17:29:06Z

<p>Jcote: </p> <hr /> <div><br /> <br /> =Extraction and Purification of DNA from Tail Biopsy=<br /> <br /> &#039;&#039;&#039;Tail Biopsy&#039;&#039;&#039;<br /> <br /> Mice are first anesthetized in a bell jar with Isoflurane for ~20 sec, monitor animal closely and check with toe pinch before proceeding<br /> <br /> <br /> =Cycling Protocol=<br /> <br /> The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele. <br /> # 95.0° for 2 min <br /> # 95.0° for 20 sec<br /> # 65.0° for 15 sec, -0.5° per cycle<br /> # 72.0° for 10 sec<br /> # Goto 2, 10 times<br /> # 95.0° for 15 sec <br /> # 60.0° for 15 sec<br /> # 72.0° for 10 sec<br /> # Goto 6, 30 times<br /> # 72.0° for 5 min<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3223 2024-07-24T17:05:43Z

<p>Jcote: </p> <hr /> <div><br /> <br /> =Extraction and Purification of DNA from Tail Biopsy=<br /> <br /> =Cycling Protocol=<br /> <br /> The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele. <br /> # 95.0° for 2 min <br /> # 95.0° for 20 sec<br /> # 65.0° for 15 sec, -0.5° per cycle<br /> # 72.0° for 10 sec<br /> # Goto 2, 10 times<br /> # 95.0° for 15 sec <br /> # 60.0° for 15 sec<br /> # 72.0° for 10 sec<br /> # Goto 6, 30 times<br /> # 72.0° for 5 min<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3222 2024-07-24T17:05:29Z

<p>Jcote: </p> <hr /> <div><br /> <br /> <br /> {{lowercase title}}<br /> <br /> =Extraction and Purification of DNA from Tail Biopsy=<br /> <br /> =Cycling Protocol=<br /> <br /> The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele. <br /> # 95.0° for 2 min <br /> # 95.0° for 20 sec<br /> # 65.0° for 15 sec, -0.5° per cycle<br /> # 72.0° for 10 sec<br /> # Goto 2, 10 times<br /> # 95.0° for 15 sec <br /> # 60.0° for 15 sec<br /> # 72.0° for 10 sec<br /> # Goto 6, 30 times<br /> # 72.0° for 5 min<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3221 2024-07-24T17:04:39Z

<p>Jcote: /* Extraction and Purification of DNA from Tail Biopsy */</p> <hr /> <div><br /> {{lowercase title}}<br /> <br /> {{lowercase title}}<br /> =Extraction and Purification of DNA from Tail Biopsy=<br /> <br /> =Cycling Protocol=<br /> <br /> The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele. <br /> # 95.0° for 2 min <br /> # 95.0° for 20 sec<br /> # 65.0° for 15 sec, -0.5° per cycle<br /> # 72.0° for 10 sec<br /> # Goto 2, 10 times<br /> # 95.0° for 15 sec <br /> # 60.0° for 15 sec<br /> # 72.0° for 10 sec<br /> # Goto 6, 30 times<br /> # 72.0° for 5 min<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3220 2024-07-24T17:03:53Z

<p>Jcote: </p> <hr /> <div><br /> {{lowercase title}}<br /> =Extraction and Purification of DNA from Tail Biopsy=<br /> <br /> <br /> <br /> <br /> <br /> {{lowercase title}}<br /> =Cycling Protocol=<br /> <br /> The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele. <br /> # 95.0° for 2 min <br /> # 95.0° for 20 sec<br /> # 65.0° for 15 sec, -0.5° per cycle<br /> # 72.0° for 10 sec<br /> # Goto 2, 10 times<br /> # 95.0° for 15 sec <br /> # 60.0° for 15 sec<br /> # 72.0° for 10 sec<br /> # Goto 6, 30 times<br /> # 72.0° for 5 min<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3219 2024-07-24T17:03:21Z

<p>Jcote: </p> <hr /> <div><br /> {{lowercase title}}<br /> <br /> =Extraction and Purification of DNA from Tail Biopsy=<br /> <br /> <br /> <br /> <br /> <br /> {{lowercase title}}<br /> <br /> =Cycling Protocol=<br /> <br /> The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele. <br /> # 95.0° for 2 min <br /> # 95.0° for 20 sec<br /> # 65.0° for 15 sec, -0.5° per cycle<br /> # 72.0° for 10 sec<br /> # Goto 2, 10 times<br /> # 95.0° for 15 sec <br /> # 60.0° for 15 sec<br /> # 72.0° for 10 sec<br /> # Goto 6, 30 times<br /> # 72.0° for 5 min<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3218 2024-07-24T16:21:21Z

<p>Jcote: </p> <hr /> <div><br /> {{lowercase title}}<br /> <br /> =Extraction and Purification of DNA from Tail Biopsy=<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3217 2024-07-24T16:18:05Z

<p>Jcote: </p> <hr /> <div>Protocol for extraction and purification of DNA from FosCreER x DREADD transgenic colony<br /> <br /> =Extraction Procedure=<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3216 2024-07-24T16:17:00Z

<p>Jcote: </p> <hr /> <div>Protocol for extraction and purification of DNA from FosCreER x DREADD transgenic colony<br /> <br /> Extraction Procedure <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3215 2024-07-24T16:16:23Z

<p>Jcote: </p> <hr /> <div>&#039;&#039;&#039;Protocol for extraction and purification of DNA from FosCreER x DREADD transgenic colony&#039;&#039;&#039;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=Protocols&diff=3214 2024-07-24T16:16:02Z

<p>Jcote: </p> <hr /> <div>Protocols used by the Houpt Lab.<br /> <br /> ==Behavioral Protocols==<br /> <br /> ==Surgical Protocols==<br /> <br /> * [[ICV Cannulation]]<br /> * [[Intraoral Catheter]]<br /> <br /> ==Histological Protocols==<br /> <br /> * [[Immunohistochemistry]]<br /> * [[In Situ Hybridization]]<br /> * [[BrdU]]<br /> * [[LacZ]] -- need to add BSK&#039;s staining protocol!<br /> <br /> ==Molecular Protocols==<br /> <br /> * [[cDNA Random Priming]]<br /> * [[Oligo Tail Labeling]]<br /> * [[RNA Extraction]] (with Trizol )<br /> * [[cDNA Blot]]<br /> * [[Laser Capture Microscopy (LCM)]]<br /> * [[Reverse Transcription]]<br /> * [[Real-Time PCR]]<br /> * [[FosCreER DREADD PCR]]<br /> <br /> A list of pages with protocols:<br /> <br /> ::[[:Category:Protocols]]<br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=FosCreER_DREADD_PCR&diff=3213 2024-07-24T16:05:58Z

<p>Jcote: Created page with &quot;Protocol for extraction and purification of DNA from FosCreER x DREADD transgenic colony Category:Protocols&quot;</p> <hr /> <div>Protocol for extraction and purification of DNA from FosCreER x DREADD transgenic colony<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3212 2024-07-19T20:38:04Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation: Dissolved in isotonic saline 0.3mg/ml shaking <br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCF<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2024-1-28<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=7 male, n=16 female)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2024-2-28)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3211 2024-07-19T19:31:30Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation: Dissolved in sterile isotonic saline 0.3mg/ml shaking <br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution. After 15 minutes mice are then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after another 15 min.<br /> <br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3210 2024-07-19T18:47:48Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation: Dissolved by shaking in sterile isotonic saline at 0.3mg/ml<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution. After 15 minutes mice are then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after another 15 min.<br /> <br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCG_Inhibitory_DREADD_Functional_Validation&diff=3187 2024-05-31T17:59:39Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCG<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2024-1-28<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCG Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2024-2-28)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCG01-05: ???<br /> * JCG06-10: ???<br /> <br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCG_Inhibitory_DREADD_Functional_Validation&diff=3186 2024-05-31T17:58:25Z

<p>Jcote: /* Data */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCF<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2024-1-28<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCG Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2024-2-28)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCG01-05: ???<br /> * JCG06-10: ???<br /> <br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCG_Inhibitory_DREADD_Functional_Validation&diff=3185 2024-05-31T17:58:10Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCF<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2024-1-28<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2024-2-28)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCG01-05: ???<br /> * JCG06-10: ???<br /> <br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCG_Inhibitory_DREADD_Functional_Validation&diff=3184 2024-05-31T17:56:55Z

<p>Jcote: Created page with &quot;==Agenda== Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-) Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at...&quot;</p> <hr /> <div>==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2024-2-28)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCG01-05: ???<br /> * JCG06-10: ???<br /> <br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3179 2024-02-27T19:36:50Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCF<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2024-1-28<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2024-2-28)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3178 2024-02-19T22:43:18Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution. After 15 minutes mice are then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after another 15 min.<br /> <br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3176 2024-02-12T20:44:38Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCF<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2024-1-28<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3175 2024-02-12T20:44:08Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCF<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3174 2024-02-12T20:43:30Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (**2024-7-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3173 2024-01-29T01:26:56Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3172 2024-01-29T01:26:42Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> 4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3171 2024-01-27T23:02:03Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/28/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min <br /> <br /> Groups: <br /> * LTC: Exp group (Predicted to form taste aversion)<br /> * VVC: Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3170 2024-01-26T17:34:46Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/27/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6: Excitatory DREADDs are expected to be expressed<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6: No DREADDs are expected to be expressed<br /> <br /> 3. Isotonic saline and 4-OHT, N=6: Due to the presence of 4-OHT but no LiCl, some DREADD expression is expected<br /> <br /> 4. Isotonic saline and sesame oil, N=5 No DREADD expression expected<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p). ***Only LVL group will receive LiCl (0.15M,20ml/kg, i.p) after 10 min <br /> <br /> Groups: <br /> * LTL: Exp group (Predicted to form taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCF01-04: ???<br /> * JCF05-08: ???<br /> * JCF09-12: ???<br /> * JCF13-16: ???<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3169 2024-01-25T21:27:27Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (1/27/24) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3168 2024-01-25T21:11:40Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3167 2024-01-25T21:11:27Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}<br /> }<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3166 2024-01-25T21:10:28Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}<br /> <br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3165 2024-01-25T21:10:03Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion. <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCF_Excitatory_DREADD_Functional_Validation&diff=3164 2024-01-25T21:05:17Z

<p>Jcote: Created page with &quot;To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. C...&quot;</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCF}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3144 2023-10-06T18:31:07Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> <br /> Groups: <br /> * LTL: Exp group (predicted to show attenuated taste aversion)<br /> * LVL: + Control (expect to form taste aversion)<br /> * VTV: - Control (expect to not form taste aversion)<br /> * VVV: - Control (expect to not form taste aversion) <br /> <br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3137 2023-09-23T22:31:40Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min<br /> <br /> <br /> Groups: All mice receive CNO &amp; LiCl injections<br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3134 2023-09-10T20:52:08Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-12)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-9-13 - 2023-9-23)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-9-24)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min<br /> <br /> <br /> Groups: All mice receive CNO &amp; LiCl injections<br /> <br /> * JCE01-04: Female<br /> * JCE05-08: Female<br /> * JCE09-12: Male<br /> * JCE13-16: Male<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-9-25 - 2023-10-6 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3132 2023-08-16T17:21:04Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment (8/18/23) 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=5<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-9-1)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-8-27 - 2023-9-06)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min<br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3131 2023-08-10T17:57:54Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min. <br /> <br /> Pre Treatment 2 weeks before starting water restriction<br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=4<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-8-26)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-8-27 - 2023-9-06)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min<br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3130 2023-08-09T23:17:03Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p), 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p), Sesame oil vehicle (10ml/kg i.p)<br /> <br /> Pre Treatment 2 weeks before starting water restriction, all injections are given concomitantly <br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=4<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-8-26)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-8-27 - 2023-9-06)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> <br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min<br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3129 2023-08-09T23:16:33Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p), 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p), Sesame oil vehicle (10ml/kg i.p)<br /> <br /> Pre Treatment 2 weeks before starting water restriction, all injections are given concomitantly <br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=4<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-8-26)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-8-27 - 2023-9-06)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min<br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3127 2023-08-08T03:43:33Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p), 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p), Sesame oil vehicle (10ml/kg i.p)<br /> <br /> Pre Treatment 2 weeks before starting water restriction, all injections are given concomitantly <br /> 4 groups:<br /> <br /> 1. Isotonic LiCl and 4-OHT, N=6<br /> <br /> 2. Isotonic LiCl and sesame oil, N=6<br /> <br /> 3. Isotonic saline and 4-OHT, N=6<br /> <br /> 4. Isotonic saline and sesame oil, N=4<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3126 2023-08-08T03:38:56Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p), 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p), Sesame oil vehicle (10ml/kg i.p)<br /> <br /> Pre Treatment 2 weeks before starting water restriction, 4 groups:<br /> <br /> 1. Isotonic LiCl (0.15M, 20ml/kg, i.p) and 4-OHT (50 mg/kg ip), N=6<br /> <br /> 2. Isotonic LiCl(0.15M, 20ml/kg, i.p) and sesame oil (10 ml/kg i.p), N=6<br /> <br /> 3. Isotonic saline (20ml/kg, i.p) and 4-OHT (50 mg/kg i.p), N=6<br /> <br /> 4. Isotonic saline (20ml/kg, i.p) and sesame oil (10ml/kg i.p), N=4<br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3125 2023-08-08T03:28:53Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Pre Treatment 2 weeks before starting water restriction, 4 groups:<br /> <br /> 1. Isotonic LiCl (0.15M, 20ml/kg, i.p) and 4-OHT (50 mg/kg ip), N=6<br /> <br /> 2. Isotonic LiCl(0.15M, 20ml/kg, i.p) and sesame oil (10 ml/kg i.p), N=6<br /> <br /> 3. Isotonic saline (20ml/kg, i.p) and 4-OHT (50 mg/kg i.p), N=6<br /> <br /> 4. Isotonic saline (20ml/kg, i.p) and sesame oil (10ml/kg i.p), N=4<br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3124 2023-08-08T03:27:16Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Pre Treatment 2 weeks before starting water restriction, 4 groups:<br /> <br /> 1. Mice will be injected with isotonic LiCl (0.15M, 20ml/kg, i.p) and 4-OHT (50 mg/kg ip), N=6<br /> <br /> 2. Mice will be injected with isotonic LiCl(0.15M,<br /> 20ml/kg, i.p) and sesame oil (10 ml/kg i.p), N=6<br /> <br /> 3. Mice will be injected with isotonic saline<br /> (20ml/kg, i.p) and 4-OHT (50 mg/kg i.p), N=6<br /> <br /> 4. Mice will be injected with isotonic saline (20ml/kg, i.p) and <br /> sesame oil (10ml/kg i.p), N=4<br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3123 2023-08-08T03:26:47Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> Pre Treatment 2 weeks before starting water restriction, 4 groups:<br /> <br /> 1. Mice will be injected with isotonic LiCl (0.15M,<br /> 20ml/kg, i.p) and 4-OHT (50 mg/kg ip), N=6<br /> <br /> 2. Mice will be injected with isotonic LiCl(0.15M,<br /> 20ml/kg, i.p) and sesame oil (10 ml/kg i.p), N=6<br /> <br /> 3. Mice will be injected with isotonic saline<br /> (20ml/kg, i.p) and 4-OHT (50 mg/kg i.p), N=6<br /> <br /> 4. Mice will be injected with isotonic saline (20ml/kg, i.p) and <br /> sesame oil (10ml/kg i.p), N=4<br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3122 2023-08-08T01:28:07Z

<p>Jcote: /* Agenda */</p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)<br /> <br /> <br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3121 2023-08-07T00:24:07Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCE<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCE<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCE Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice JAX C57BL/6J Strain 000664 (n=8 male DOB +/- 3: 20Dec2022, n=8 female DOB +/- 3: 20Dec2022)<br /> <br /> Arrived 2023-2-6.<br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3120 2023-08-07T00:23:26Z

<p>Jcote: </p> <hr /> <div>To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion. <br /> <br /> <br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCD<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCD<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCD Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice JAX C57BL/6J Strain 000664 (n=8 male DOB +/- 3: 20Dec2022, n=8 female DOB +/- 3: 20Dec2022)<br /> <br /> Arrived 2023-2-6.<br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote https://wiki.houptlab.org/index.php?title=JCE_Inhibitory_DREADD_Functional_Validation&diff=3119 2023-08-06T20:32:44Z

<p>Jcote: </p> <hr /> <div>To test CNO as a uncondition stimulus for CTA in wild-type mice.<br /> <br /> <br /> CNO ordering information / lot numbers<br /> <br /> CNO preparation (e.g. vehicle, how its dissolved)<br /> <br /> {{Infobox experiment|align=right<br /> | Name = Clozapine-N-Oxide Taste Aversion<br /> | project = DREADDs and Magentic Field<br /> | code = JCD<br /> | protocol = 202200000006<br /> | funding = Chair&#039;s Fund<br /> | investigators = Cote, Houpt<br /> | species = mice<br /> | date = 2023-6-19<br /> | type = CTA<br /> | drugs = NaCl, CNO<br /> | artifacts = <br /> | data = https://houptlab.org/bartab/expt/?id=JCD<br /> }}<br /> <br /> <br /> ==Data== <br /> <br /> &#039;&#039;&#039;[https://houptlab.org/bartab/expt/?id=JCD Raw Data Link]&#039;&#039;&#039;<br /> <br /> ==People== <br /> <br /> Jason Cote<br /> <br /> ==Agenda==<br /> <br /> Mice JAX C57BL/6J Strain 000664 (n=8 male DOB +/- 3: 20Dec2022, n=8 female DOB +/- 3: 20Dec2022)<br /> <br /> Arrived 2023-2-6.<br /> <br /> &#039;&#039;&#039;Day 0: Water removed&#039;&#039;&#039; (2023-6-19)<br /> <br /> <br /> &#039;&#039;&#039;Days 1 - 11: Water Restriction&#039;&#039;&#039; (2023-6-20 - 2023-6-30)<br /> <br /> Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min. <br /> <br /> <br /> <br /> Mice given water bottle and empty bottle, alternating position. <br /> <br /> In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.<br /> &#039;&#039;&#039;Day 12: Conditioning Day&#039;&#039;&#039; (2023-3-16)<br /> <br /> Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg <br /> <br /> <br /> Groups: <br /> <br /> * JCC01-04: Female, vehicle<br /> * JCC05-08: Female, CNO<br /> * JCC09-12: Male, vehicle<br /> * JCC13-16: Male, CNO<br /> <br /> After injection, ad lib water returned overnight.<br /> <br /> &#039;&#039;&#039;Day 13 - 24: Preference Tests&#039;&#039;&#039; (2023-3-17 - 2023-3-24 )<br /> <br /> CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.<br /> <br /> <br /> [[Category:Taste Aversion]]<br /> [[Category:CTA Experiments]]<br /> [[Category:Magnet Experiments]]</div>

Jcote