https://wiki.houptlab.org/api.php?action=feedcontributions&feedformat=atom&user=KimbroughMagnetoWiki - User contributions [en]2026-04-07T13:15:47ZUser contributionsMediaWiki 1.35.8https://wiki.houptlab.org/index.php?title=Neurogenesis&diff=1825Neurogenesis2008-11-06T19:41:38Z<p>Kimbrough: /* Useful Markers */</p>
<hr />
<div>=Useful Markers=<br />
<br />
'''Ki67''' <br />
<br />
marker for mitotic cells<br />
<br />
a rabbit anti-rat Ki67 monoclonal antibody (1:750, Biomeda, Foster City, CA) From Corniola et al PMID 18778998<br />
<br />
<blockquote><br />
The nuclear protein Ki67 is expressed during all active phases of the cell cycle. Three weeks of dietary zinc deficiency reduced the number of Ki67-positive cells in the SGZ and GCL of the dentate, suggesting that zinc is required for neuronal precursor cell proliferation in vivo. This conclusion is supported by the finding that zinc restriction of human neuronal precursors in culture decreased the number of cells that entered S-phase, as evidenced by a reduction in BrdU labeling.<br />
</blockquote><br />
<br />
<br />
'''GAD67 (or was it 65?)'''<br />
<br />
anti-GAD67 rabbit antibody (1/1,000 for Alexa 594-conjugated streptavidin detection, 1/500,000 for TSA detection; (Chemicon) From Tamamaki N et al PMID: 14574680<br />
<br />
Look up references by Leo the olfactory fellow from NIH<br />
<br />
'''Doublecortin'''<br />
<br />
marker for migrating cells DCX (Santa Cruz) From Imayoashi et al PMID: 18758458<br />
<br />
'''NeuroTrace'''<br />
<br />
Molecular Probes from Bagley et al PMID: 17996088</div>Kimbroughhttps://wiki.houptlab.org/index.php?title=Neurogenesis&diff=1824Neurogenesis2008-11-06T19:41:05Z<p>Kimbrough: /* Useful Markers */</p>
<hr />
<div>=Useful Markers=<br />
<br />
'''Ki67''' <br />
<br />
marker for mitotic cells<br />
<br />
a rabbit anti-rat Ki67 monoclonal antibody (1:750, Biomeda, Foster City, CA) From Corniola et al PMID 18778998<br />
<br />
<blockquote><br />
The nuclear protein Ki67 is expressed during all active phases of the cell cycle. Three weeks of dietary zinc deficiency reduced the number of Ki67-positive cells in the SGZ and GCL of the dentate, suggesting that zinc is required for neuronal precursor cell proliferation in vivo. This conclusion is supported by the finding that zinc restriction of human neuronal precursors in culture decreased the number of cells that entered S-phase, as evidenced by a reduction in BrdU labeling.<br />
</blockquote><br />
<br />
<br />
'''GAD67 (or was it 65?)'''<br />
<br />
anti-GAD67 rabbit antibody (1/1,000 for Alexa 594-conjugated streptavidin detection, 1/500,000 for TSA detection; Chemicon From Tamamaki N et al PMID: 14574680<br />
<br />
Look up references by Leo the olfactory fellow from NIH<br />
<br />
'''Doublecortin'''<br />
<br />
marker for migrating cells DCX (Santa Cruz) From Imayoashi et al PMID: 18758458<br />
<br />
'''NeuroTrace'''<br />
<br />
Molecular Probes from Bagley et al PMID: 17996088</div>Kimbroughhttps://wiki.houptlab.org/index.php?title=Neurogenesis&diff=1823Neurogenesis2008-11-06T19:33:42Z<p>Kimbrough: /* Useful Markers */</p>
<hr />
<div>=Useful Markers=<br />
<br />
'''Ki67''' <br />
<br />
marker for mitotic cells<br />
<br />
a rabbit anti-rat Ki67 monoclonal antibody (1:750, Biomeda, Foster City, CA) From Corniola et al PMID 18778998<br />
<br />
<blockquote><br />
The nuclear protein Ki67 is expressed during all active phases of the cell cycle. Three weeks of dietary zinc deficiency reduced the number of Ki67-positive cells in the SGZ and GCL of the dentate, suggesting that zinc is required for neuronal precursor cell proliferation in vivo. This conclusion is supported by the finding that zinc restriction of human neuronal precursors in culture decreased the number of cells that entered S-phase, as evidenced by a reduction in BrdU labeling.<br />
</blockquote><br />
<br />
<br />
'''GAD67 (or was it 65?)'''<br />
<br />
Look up references by Leo the olfactory fellow from NIH<br />
<br />
'''Doublecortin'''<br />
<br />
marker for migrating cells DCX (Santa Cruz) From Imayoashi et al PMID: 18758458<br />
<br />
'''NeuroTrace'''<br />
<br />
Molecular Probes from Bagley et al PMID: 17996088</div>Kimbrough