View source for DMX Orchiectomy and Butyrate-Enhanced CTA

Test of role of testicular hormones in butyrate-enhanced CTA.

Mukherjee showed that HDAC inhibitors butyrate and calproate enchanced CTA in male but not female rats.  Ovariectomy did not make female rats sensitive, so testing if orchiectomy reduces sensitivity of male rats.


{{Infobox experiment|align=right
| Name = Orchiectomy and Butyrate-Enhanced CTA
| project = HDAC Sex Difference
| code = DMX
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Mukeherjee, Bass, Houpt
| species = rat
| date = 2024-6-19
| type = CTA
| drugs = butyrate, lithium
| artifacts = 
| data = https://houptlab.org/bartab/expt/?id=DMX
}}

Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]

* 0.15 M LiCl = 3.18g / 500ml water
* 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385)
* 0.3 M NaCl = 1.8g/100ml water (osmotic control)

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