View source for DMC Sodium Butyrate and Flavor Conditioning


Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances preference learning 

Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] 

==Data== 

'''[https://houptlab.org/bartab/expt/?id=DMC Raw Data Link]'''

==People== 

Durba Mukherjee, Alex Haimovich

==Agenda==

n=16 adult male sprague-dawley rats (Charles River CD), individually housed in BRF 169

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'''Days 0-8: Food and water restricted.''' (October 8 - October 16, 2019)

Weigh rats daily.  water for 30 min, food 3-4 pellets to maintain at 90% body weight. 

Water removed on Tuesday Oct 8, 2019. 30 min water at 1pm daily, pellet ration at 1:30pm

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'''Day 9: Conditioning Day 1''' (October 17, 2019)

0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)

30 min: remove CS+, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, give daily pellet ration

* Rats 1-8: grape CS+, Rats 9-16: Cherry CS+.
* Odd rats: NaCL injection. Even rats: NaB injection

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'''Day 10: Conditioning Day 2''' (October 18, 2019)

0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape)

30 min: remove CS-, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, return food and water ad lib

* Rats 1-8: Cherry CS-, Rats 9-16: Grape CS-.
* Odd rats: NaCL injection. Even rats: NaB injection

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'''Days 11-17: 2 bottle preference tests''' (October 19-25, 2019)

Each rat given adlib 24-h access to of CS+/sac vs CS-/sac.  Position of bottle alternated each day.

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[[Category:Flavor Preference]]