View source for DMR Ovariectomy and Butyrate-Enhanced CTA 2


Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of [[DMP Ovariectomy and Butyrate-Enhanced CTA]].

NOTE: concerned about low bodyweight of rats, amd that butyrate was not freshly made.

{{Infobox experiment|align=right
| Name = Ovariectomy and Butyrate-Enhanced CTA 2
| project = HDAC Sex Difference
| code = DMR
| protocol = 1917
| funding = Chair's Fund
| investigators = Mukherjee, Houpt
| species = rat
| date = 2022-1-9
| type = CTA
| drugs = butyrate, lithium, estradiol
| artifacts = 
| data = https://houptlab.org/bartab/expt/?id=DMR
}}

Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] 

* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)

<br>

==Data== 

'''[https://houptlab.org/bartab/expt/?id=DMR Raw Data Link]'''

==People== 

Durba Mukherjee



===Agenda===

'''Rats arrive''' (Charles River 2021-12-7)
Female Sprague Dawley rats n=13 (dob 2021-9-20 


<br>
'''Surgery'''

3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)

* DMR 01-06: Surgery on 2021-12-30
* DMR 07-12: Surgery on 2021-12-31
* DMR 13: Surgery on 2022-1-2

Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.


'''Day 0: Water removed 2022-1-09'''

<br>

'''Days 1-7: Water Restriction 2022-1-9 thru 2022-1-17'''

Weigh rats daily.  Water for 10 min, ad lib food. 

10 min water at 1400 daily.

<br>

'''Day 8: Conditioning Day 2022-1-18'''

* 0-30 min: saccharin 0.125%

* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)

* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl

ad lib water returned after final injection overnight.

Groups:

* Sham + NaCl: DMR04,DMR06,DMR10,DMR12
* Sham + Butyrate: DMR05,DMR11,DMR13
* OVX + NaCl: DMR01, DMR03,DMR08
* OVX + Butyrate: DMR02,FMR07,DMR09

<br>

'''Days 9 - 23: Preference Tests: 2022-1-19 to ????'''

Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.


[[Category:Taste Aversion]]
[[Category: CTA Experiments]]
[[Category: Epigenetics]]