Difference between revisions of "Bipolar Genes"
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primers. For G72 gene forward: 5'-GCTGAATGAGAAGCA-3'; reverse: 5'-TGGGTCCCAGACACAGAGT-3'; | primers. For G72 gene forward: 5'-GCTGAATGAGAAGCA-3'; reverse: 5'-TGGGTCCCAGACACAGAGT-3'; | ||
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Latest revision as of 22:09, 23 February 2009
RT-PCR Conditions for a bunch of genes implicated in Bipolar Disorder
DAOA (D-amino acid oxidase activator, aka G72)
DISC1 (disrupted in schizophrenia 1)
NRG1 (neuregulin1)
DTNBP1 (dystrobrevin binding protein 1)
PALB2 (partner and localizer of BRCA2)
NDUFAB1 (NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1)
DCTN5 (dynactin 5)
DAAO
Source: Konno 1998 PMID 12734589
Sense primer (F: 5’-GGTTAACTGAGAGGGGAGTGAA-3’)
Antisense primer (R: 5’- CCATAGTTGTGGATGACCTCTG-3’)
designed from the sequence conserved in cDNAs encoding human, mouse, rabbit, and pig D-amino-acid oxidases.
initial denaturation at 94°C for 1 min, 30 cycles of PCR
- denaturation: 94°C, 30 sec
- annealing: 55°C, 30 sec
- extension: 72°C, 45 sec)
final extension was done at 72°C for 5 min
Serine Racemase
See entry for Serine Racemase
DAOA
Source: Korostishevsky 2004 PMID 15271585
primers. For G72 gene forward: 5'-GCTGAATGAGAAGCA-3'; reverse: 5'-TGGGTCCCAGACACAGAGT-3';