Difference between revisions of "DMC Sodium Butyrate and Flavor Conditioning"
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| + | Test if systemic Sodium Butyrate, an HDAC inhibitor, enhances preference learning | ||
| − | + | ==People== | |
| − | |||
| − | + | Durba Mukherjee, Alex Haimovich | |
| − | |||
| − | |||
| − | |||
| − | + | ==Agenda== | |
| − | |||
| − | |||
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| − | + | n=16 adult male sprague-dawley rats (Charles River CD), individually housed in BRF 169 | |
| + | <br> | ||
| + | '''Days 0-8: Food and water restricted.''' (October 8 - October 16, 2019) | ||
| + | Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight. | ||
| + | |||
| + | Water removed on Tuesday Oct 8, 2019. 30 min water at 1pm daily, pellet ration at 1:30pm | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Day 9: Conditioning Day 1''' (October 17, 2019) | ||
| + | |||
| + | 0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape) | ||
| + | |||
| + | 30 min: remove CS+, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, give daily pellet ration | ||
| + | |||
| + | * Rats 1-8: grape CS+, Rats 9-16: Cherry CS+. | ||
| + | * Odd rats: NaCL injection. Even rats: NaB injection | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Day 10: Conditioning Day 1''' (October 18, 2019) | ||
| + | |||
| + | 0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape) | ||
| + | |||
| + | 30 min: remove CS-, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, return food and water ad lib | ||
| + | |||
| + | * Rats 1-8: Cherry CS-, Rats 9-16: Grape CS-. | ||
| + | * Odd rats: NaCL injection. Even rats: NaB injection | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Days 11-17: 2 bottle preference tests''' (October 19-25, 2019) | ||
| + | |||
| + | Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day. | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <br> | ||
[[Category:Flavor Preference]] | [[Category:Flavor Preference]] | ||
Revision as of 09:51, 17 October 2019
Test if systemic Sodium Butyrate, an HDAC inhibitor, enhances preference learning
People
Durba Mukherjee, Alex Haimovich
Agenda
n=16 adult male sprague-dawley rats (Charles River CD), individually housed in BRF 169
Days 0-8: Food and water restricted. (October 8 - October 16, 2019)
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
Water removed on Tuesday Oct 8, 2019. 30 min water at 1pm daily, pellet ration at 1:30pm
Day 9: Conditioning Day 1 (October 17, 2019)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)
30 min: remove CS+, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, give daily pellet ration
- Rats 1-8: grape CS+, Rats 9-16: Cherry CS+.
- Odd rats: NaCL injection. Even rats: NaB injection
Day 10: Conditioning Day 1 (October 18, 2019)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)
30 min: remove CS-, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, return food and water ad lib
- Rats 1-8: Cherry CS-, Rats 9-16: Grape CS-.
- Odd rats: NaCL injection. Even rats: NaB injection
Days 11-17: 2 bottle preference tests (October 19-25, 2019)
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.