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Line 8:
Line 8: − + + − Conditioning Days:+ + + + + − Day 1: 30 min access to CS+/glucose = 0.2% Koolaid + 0.2% saccharin + either 0% glucose, or 4% glucose, or 8% glucose+ − Day 2: 30 min access to CS-/glucose = 0.2% Koolaid + 0.2% saccharin − + + + + + + + + + +
DMD Conditioned Flavor Preference Glucose Dose (view source)
Revision as of 13:40, 4 February 2020
, 13:40, 4 February 2020→Agenda
n=18 male and female rats (TH-GFP rats from Trombley).
n=18 male and female rats (TH-GFP rats from Trombley).
Baseline: Placed on food and water restriction: Rats weighed daily, given 30 min access to water vs saccharin from 130pm to 2pm, then given measured ration of chow to maintain 90% initial bodyweight
===Baseline===
* Placed on food and water restriction
Daily routine:
* Rats weighed.
* give measured ration of chow to maintain 90% initial bodyweight
* given 30 min access to water vs 8% maltodextin (2 bottles) from 2pm to 230pm
* bottle sides alternated each day
Continue baseline until latency to drink and intakes are stabilized.
Two bottle preference tests of CS+/saccahrin vs CS-/saccharin
Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight
===Conditioning Days===
* CS+ = 0.2% Koolaid + 0.033% saccharin
* US = glucose at 0, 4 or 8%
Day 1: 30 min access to CS+/glucose = 0.2% Koolaid + 0.033% saccharin + either 0% glucose, or 4% glucose, or 8% glucose
Day 2: Two bottle preference tests of CS+ vs water are begun