Difference between revisions of "DMD Conditioned Flavor Preference Glucose Dose"

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(Created page with " Investigators: Mukherjee, Houpt Protocol: 1917 ==Agenda== n=18 male and female rats (TH-GFP rats from Trombley). Baseline: Placed on food and water restriction: Rats weig...")
 
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n=18 male and female rats (TH-GFP rats from Trombley).
 
n=18 male and female rats (TH-GFP rats from Trombley).
  
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Baseline: Placed on food and water restriction: Rats weighed daily, given 30 min access to water vs saccharin from 130pm to 2pm, then given measured ration of chow to maintain 90% initial bodyweight
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===Baseline===
 +
* Placed on food and water restriction
  
−
Conditioning Days:
+
Daily routine:
 +
* Rats weighed.
 +
* give measured ration of chow to maintain 90% initial bodyweight
 +
* given 30 min access to water vs 8% maltodextin (2 bottles) from 2pm to 230pm
 +
* bottle sides alternated each day
  
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Day 1: 30 min access to CS+/glucose = 0.2% Koolaid + 0.2% saccharin + either 0% glucose, or 4% glucose, or 8% glucose
+
Continue baseline until latency to drink and intakes are stabilized.
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Day 2: 30 min access to CS-/glucose = 0.2% Koolaid + 0.2% saccharin
 
  
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Two bottle preference tests of CS+/saccahrin vs CS-/saccharin
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Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight
 +
 
 +
===Conditioning Days===
 +
 
 +
* CS+ = 0.2% Koolaid + 0.033% saccharin
 +
* US = glucose at 0, 4 or 8%
 +
 
 +
Day 1: 30 min access to CS+/glucose = 0.2% Koolaid + 0.033% saccharin + either 0% glucose, or 4% glucose, or 8% glucose
 +
 
 +
Day 2: Two bottle preference tests of CS+ vs water are begun

Revision as of 12:40, 4 February 2020

Investigators: Mukherjee, Houpt

Protocol: 1917

Agenda

n=18 male and female rats (TH-GFP rats from Trombley).

Baseline

  • Placed on food and water restriction

Daily routine:

  • Rats weighed.
  • give measured ration of chow to maintain 90% initial bodyweight
  • given 30 min access to water vs 8% maltodextin (2 bottles) from 2pm to 230pm
  • bottle sides alternated each day

Continue baseline until latency to drink and intakes are stabilized.

Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight

Conditioning Days

  • CS+ = 0.2% Koolaid + 0.033% saccharin
  • US = glucose at 0, 4 or 8%

Day 1: 30 min access to CS+/glucose = 0.2% Koolaid + 0.033% saccharin + either 0% glucose, or 4% glucose, or 8% glucose

Day 2: Two bottle preference tests of CS+ vs water are begun

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