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Line 28: − + + + + − * CS+ = 0.2% Koolaid + 0.033% saccharin + + + + + + + − Day 1: 30 min access to CS+/glucose = 0.2% Koolaid + 0.033% saccharin + either 0% glucose, or 4% glucose, or 8% glucose+ − Day 2: Two bottle preference tests of CS+ vs water are begun+ + + + + + + + +
DMD Conditioned Flavor Preference Glucose Dose (view source)
Revision as of 12:08, 11 February 2020
, 12:08, 11 February 2020fleshed out protocol
==Purpose==
==Purpose==
Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Koolaid + 0.033% saccharin, based on Golden data from experiment GI).
Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Koolaid + 0.05% saccharin, based on Golden data from experiment GI -- see chapter 3 of Golden's dissertation).
Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate).
Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate).
==Agenda==
==Agenda==
n=18 male and female rats (TH-GFP rats from Trombley).
n=18 male and female rats (TH-GFP rats from Trombley). Male and female alternate even and odd id numbers (CHECK which is which). 3 groups: 0% glucose US (rats DMD01-06), 4% glucose US (rats DMD07-12), and 8% glucose US (rats DMD13-18)
===Baseline===
===Baseline===
* Placed on food and water restriction
* Placed on food and water restriction
* Rats weighed.
* Rats weighed.
* give measured ration of chow to maintain 90% initial bodyweight
* give measured ration of chow to maintain 90% initial bodyweight
* given 30 min access to water vs 8% maltodextin (2 bottles) from 2pm to 230pm
* given 30 min access to water vs 8% maltodextrin (2 bottles) from 2pm to 230pm
* bottle sides alternated each day
* bottle sides alternated each day
Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight
Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight
===Conditioning Days===
===Conditioning Day===
* CS+ = 0.2% Koolaid + 0.05% saccharin
** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin
* US = glucose at 0, 4 or 8%
* US = glucose at 0, 4 or 8%
** 4% = 80g glucose / 2 liters
** 8% = 160g glucose / 2 liters
Conditioning Procedure:
* Weigh rats.
* 30 min access to CS+/glucose = 0.2% Koolaid + 0.05% saccharin + either 0% glucose, or 4% glucose, or 8% glucose.
* At end of 30 min access, return ad lib food and (weighed) water.
===Extinction Tests===
24 h after conditioning, Two Bottle Preference Tests of CS+ vs water are begun for at least 6 (?) days
* Weigh rats.
* 2 bottles with 24 h access:
** bottles weighed and refilled daily
** water
** CS+ = 0.2% Koolaid + 0.05% saccharin
*** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin
* alternate bottle sides each day
* ad lib access to chow throughout