Difference between revisions of "DMD Conditioned Flavor Preference Glucose Dose"
(fleshed out protocol) |
(fixed kool-aid concentration -> 0.05%) |
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==Purpose== | ==Purpose== | ||
| − | Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water ( | + | Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Kool-Aid + 0.05% saccharin, based on Golden data from experiment GI -- see chapter 3 of Golden's dissertation). |
Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate). | Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate). | ||
| Line 30: | Line 30: | ||
===Conditioning Day=== | ===Conditioning Day=== | ||
| − | * CS+ = 0. | + | * CS+ = 0.05% Kool-Aid + 0.05% saccharin |
| − | ** in 4 liters, | + | ** in 4 liters, 2 g Cherry Kool-Aid + 2 g sodium saccharin |
* US = glucose at 0, 4 or 8% | * US = glucose at 0, 4 or 8% | ||
| Line 39: | Line 39: | ||
Conditioning Procedure: | Conditioning Procedure: | ||
* Weigh rats. | * Weigh rats. | ||
| − | * 30 min access to CS+/glucose = 0. | + | * 30 min access to CS+/glucose = 0.05% Kool-Aid + 0.05% saccharin + either 0% glucose, or 4% glucose, or 8% glucose. |
* At end of 30 min access, return ad lib food and (weighed) water. | * At end of 30 min access, return ad lib food and (weighed) water. | ||
| Line 49: | Line 49: | ||
** bottles weighed and refilled daily | ** bottles weighed and refilled daily | ||
** water | ** water | ||
| − | ** CS+ = 0. | + | ** CS+ = 0.05% Kool-Aid + 0.05% saccharin |
*** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin | *** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin | ||
* alternate bottle sides each day | * alternate bottle sides each day | ||
* ad lib access to chow throughout | * ad lib access to chow throughout | ||
Revision as of 14:30, 12 February 2020
Investigators: Mukherjee, Houpt
Protocol: 1917
Purpose
Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Kool-Aid + 0.05% saccharin, based on Golden data from experiment GI -- see chapter 3 of Golden's dissertation).
Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate).
Agenda
n=18 male and female rats (TH-GFP rats from Trombley). Male and female alternate even and odd id numbers (CHECK which is which). 3 groups: 0% glucose US (rats DMD01-06), 4% glucose US (rats DMD07-12), and 8% glucose US (rats DMD13-18)
Baseline
- Placed on food and water restriction
Daily routine:
- Rats weighed.
- give measured ration of chow to maintain 90% initial bodyweight
- given 30 min access to water vs 8% maltodextrin (2 bottles) from 2pm to 230pm
- bottle sides alternated each day
Continue baseline until latency to drink and intakes are stabilized.
Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight
Conditioning Day
- CS+ = 0.05% Kool-Aid + 0.05% saccharin
- in 4 liters, 2 g Cherry Kool-Aid + 2 g sodium saccharin
- US = glucose at 0, 4 or 8%
- 4% = 80g glucose / 2 liters
- 8% = 160g glucose / 2 liters
Conditioning Procedure:
- Weigh rats.
- 30 min access to CS+/glucose = 0.05% Kool-Aid + 0.05% saccharin + either 0% glucose, or 4% glucose, or 8% glucose.
- At end of 30 min access, return ad lib food and (weighed) water.
Extinction Tests
24 h after conditioning, Two Bottle Preference Tests of CS+ vs water are begun for at least 6 (?) days
- Weigh rats.
- 2 bottles with 24 h access:
- bottles weighed and refilled daily
- water
- CS+ = 0.05% Kool-Aid + 0.05% saccharin
- in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin
- alternate bottle sides each day
- ad lib access to chow throughout