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DMD Conditioned Flavor Preference Glucose Dose (view source)
Revision as of 14:30, 12 February 2020
, 14:30, 12 February 2020fixed kool-aid concentration -> 0.05%
==Purpose==
==Purpose==
Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Koolaid + 0.05% saccharin, based on Golden data from experiment GI -- see chapter 3 of Golden's dissertation).
Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Kool-Aid + 0.05% saccharin, based on Golden data from experiment GI -- see chapter 3 of Golden's dissertation).
Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate).
Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate).
===Conditioning Day===
===Conditioning Day===
* CS+ = 0.2% Koolaid + 0.05% saccharin
* CS+ = 0.05% Kool-Aid + 0.05% saccharin
** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin
** in 4 liters, 2 g Cherry Kool-Aid + 2 g sodium saccharin
* US = glucose at 0, 4 or 8%
* US = glucose at 0, 4 or 8%
Conditioning Procedure:
Conditioning Procedure:
* Weigh rats.
* Weigh rats.
* 30 min access to CS+/glucose = 0.2% Koolaid + 0.05% saccharin + either 0% glucose, or 4% glucose, or 8% glucose.
* 30 min access to CS+/glucose = 0.05% Kool-Aid + 0.05% saccharin + either 0% glucose, or 4% glucose, or 8% glucose.
* At end of 30 min access, return ad lib food and (weighed) water.
* At end of 30 min access, return ad lib food and (weighed) water.
** bottles weighed and refilled daily
** bottles weighed and refilled daily
** water
** water
** CS+ = 0.2% Koolaid + 0.05% saccharin
** CS+ = 0.05% Kool-Aid + 0.05% saccharin
*** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin
*** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin
* alternate bottle sides each day
* alternate bottle sides each day
* ad lib access to chow throughout
* ad lib access to chow throughout