Difference between revisions of "DMD Conditioned Flavor Preference Glucose Dose"
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Protocol: 1917 | Protocol: 1917 | ||
| + | |||
| + | ==Purpose== | ||
| + | |||
| + | Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Kool-Aid + 0.05% saccharin, based on Golden data from experiment GI -- see chapter 3 of Golden's dissertation). | ||
| + | |||
| + | Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate). | ||
| + | |||
| + | == Online Data == | ||
| + | |||
| + | [https://houptlab.org/bartab/expt/?id=DMD Expt DMD] | ||
==Agenda== | ==Agenda== | ||
| − | n=18 male and female rats (TH-GFP rats from Trombley). | + | n=18 male and female rats (TH-GFP rats from Trombley). Male and female alternate even and odd id numbers (CHECK which is which). 3 groups: 0% glucose US (rats DMD01-06), 4% glucose US (rats DMD07-12), and 8% glucose US (rats DMD13-18) |
| + | |||
| + | ===Baseline=== | ||
| + | |||
| + | * Placed on food and water restriction | ||
| + | |||
| + | Daily routine: | ||
| + | * Rats weighed. | ||
| + | * give measured ration of chow to maintain 90% initial bodyweight | ||
| + | * given 30 min access to water vs 8% maltodextrin (2 bottles) from 2pm to 230pm | ||
| + | * bottle sides alternated each day | ||
| + | |||
| + | Continue baseline until latency to drink and intakes are stabilized. | ||
| + | |||
| + | Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight | ||
| + | |||
| + | ===Conditioning Day=== | ||
| + | |||
| + | * CS+ = 0.05% Kool-Aid + 0.05% saccharin | ||
| + | ** in 4 liters, 2 g Cherry Kool-Aid + 2 g sodium saccharin | ||
| + | |||
| + | * US = glucose at 0, 4 or 8% | ||
| + | ** 4% = 80g glucose / 2 liters | ||
| + | ** 8% = 160g glucose / 2 liters | ||
| + | |||
| + | Conditioning Procedure: | ||
| + | * Weigh rats. | ||
| + | * 30 min access to CS+/glucose = 0.05% Kool-Aid + 0.05% saccharin + either 0% glucose, or 4% glucose, or 8% glucose. | ||
| + | * At end of 30 min access, return ad lib food and (weighed) water. | ||
| − | + | ===Extinction Tests=== | |
| − | + | 24 h after conditioning, Two Bottle Preference Tests of CS+ vs water are begun for at least 6 (?) days | |
| + | * Weigh rats. | ||
| + | * 2 bottles with 24 h access: | ||
| + | ** bottles weighed and refilled daily | ||
| + | ** water | ||
| + | ** CS+ = 0.05% Kool-Aid + 0.05% saccharin | ||
| + | *** in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin | ||
| + | * alternate bottle sides each day | ||
| + | * ad lib access to chow throughout | ||
| − | + | ==Actual Timeline== | |
| − | |||
| − | + | Rats transferred to BRF. | |
| + | 2020-2-4 Rats weighed daily. Rats food and water deprived. | ||
| + | 2020-2-5 limited access to maltodextrin and water started | ||
| + | 2 | ||
| + | [[Category:Flavor Preference]] | ||
Latest revision as of 11:35, 7 July 2020
Investigators: Mukherjee, Houpt
Protocol: 1917
Purpose
Attempt single-bottle single trial conditioning using a CS+ which is iso-preferred to water (Kool-Aid + 0.05% saccharin, based on Golden data from experiment GI -- see chapter 3 of Golden's dissertation).
Will also characterize a US dose response using 0,4, or 8% glucose to determine a range of conditioning strengths (since KA + 0.2% saccharin + 8% glucose looks too strong to see any modulation by eg sodium buytrate).
Online Data
Agenda
n=18 male and female rats (TH-GFP rats from Trombley). Male and female alternate even and odd id numbers (CHECK which is which). 3 groups: 0% glucose US (rats DMD01-06), 4% glucose US (rats DMD07-12), and 8% glucose US (rats DMD13-18)
Baseline
- Placed on food and water restriction
Daily routine:
- Rats weighed.
- give measured ration of chow to maintain 90% initial bodyweight
- given 30 min access to water vs 8% maltodextrin (2 bottles) from 2pm to 230pm
- bottle sides alternated each day
Continue baseline until latency to drink and intakes are stabilized.
Notes: use maldodextrin to encourage consumption, and 2 bottles to train to make choice. Start chow ration at 25g, then adjust to maintain ~90% of baseline weight
Conditioning Day
- CS+ = 0.05% Kool-Aid + 0.05% saccharin
- in 4 liters, 2 g Cherry Kool-Aid + 2 g sodium saccharin
- US = glucose at 0, 4 or 8%
- 4% = 80g glucose / 2 liters
- 8% = 160g glucose / 2 liters
Conditioning Procedure:
- Weigh rats.
- 30 min access to CS+/glucose = 0.05% Kool-Aid + 0.05% saccharin + either 0% glucose, or 4% glucose, or 8% glucose.
- At end of 30 min access, return ad lib food and (weighed) water.
Extinction Tests
24 h after conditioning, Two Bottle Preference Tests of CS+ vs water are begun for at least 6 (?) days
- Weigh rats.
- 2 bottles with 24 h access:
- bottles weighed and refilled daily
- water
- CS+ = 0.05% Kool-Aid + 0.05% saccharin
- in 4 liters, 8 g Cherry Kool-Aid + 2 g sodium saccharin
- alternate bottle sides each day
- ad lib access to chow throughout
Actual Timeline
Rats transferred to BRF. 2020-2-4 Rats weighed daily. Rats food and water deprived. 2020-2-5 limited access to maltodextrin and water started 2