Difference between revisions of "MBC Selenate and CFP 3"
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This is the third replicate of selenate pre-Rx before glucose-induced flavor preference conditioning | This is the third replicate of selenate pre-Rx before glucose-induced flavor preference conditioning | ||
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| + | == Selenate Dosage == | ||
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| + | To test selenate attenuation of CFNP, rats will be injected with 1 ml/kg of 0.5 mg/ml Na2SeO3 in 0.15 M NaCl, (so 0.5 mg/kg selenate), 2 hours before conditioning. | ||
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| + | * Sodium Selenate Na2SeO3 from 2021-2-16 from [https://www.sigmaaldrich.com/catalog/product/sial/71950?lang=en®ion=US&gclid=EAIaIQobChMI0Lqo_9S38AIVWW5vBB0u2g1xEAAYASAAEgJ-9fD_BwE Millipore-Sigma S8295-25G] | ||
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| + | * MW 172.94, CAS Number 10102-18-8 , [https://pubchem.ncbi.nlm.nih.gov/substance/329763654 PubChem Substance ID 329763654] | ||
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| + | * 0.5 mg/ml Na2Se03 is 2.89 mM, or 8.67 mOsm (assuming complete dissociation into 2 Na+ and 1 SeO3- ions) | ||
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| + | * So OK to mix 0.5 mg Na2Se03 in 1 ml of 0.15 M NaCl, resulting in a 309 mOsm solution that is still near isotonic (300mOsM). | ||
Revision as of 09:41, 7 May 2021
This is the third replicate of selenate pre-Rx before glucose-induced flavor preference conditioning
Selenate Dosage
To test selenate attenuation of CFNP, rats will be injected with 1 ml/kg of 0.5 mg/ml Na2SeO3 in 0.15 M NaCl, (so 0.5 mg/kg selenate), 2 hours before conditioning.
- Sodium Selenate Na2SeO3 from 2021-2-16 from Millipore-Sigma S8295-25G
- MW 172.94, CAS Number 10102-18-8 , PubChem Substance ID 329763654
- 0.5 mg/ml Na2Se03 is 2.89 mM, or 8.67 mOsm (assuming complete dissociation into 2 Na+ and 1 SeO3- ions)
- So OK to mix 0.5 mg Na2Se03 in 1 ml of 0.15 M NaCl, resulting in a 309 mOsm solution that is still near isotonic (300mOsM).
Data
People
Marena Bass
Agenda
Day 0 (04/29/2021): At 5pm Rats were weighed to determine their initial body weight, and their food and water was removed to begin the food and water restriction schedule.
Days 1-8 (04/30/2021-05/07/2021) Food/water restriction and training: Adult male Sprague-Dawley rats (N=16) were trained on a restricted food and water schedule for 8 days before conditioning. Each day at 1pm, the animals were weighed and given 23-25 grams of chow to maintain 90% body weight. After being weighed, animals were given access to two bottles, 0.05% saccharin and water. Initially, they were given 1hr to drink the solutions, and this was gradually reduced to 30 mins per day. Bottle positions were alternated each day.
Day 9 (05/08/2021): CS+ Conditioning
Day 10 (05/09/2021): CS- Conditioning
Days 11-? (05/10/2021-?): 2-bottle preference testing