Difference between revisions of "DML Lower Valproate and Saccharin CTA"
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8 ml/kg = 20 mg/kg | 8 ml/kg = 20 mg/kg | ||
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| + | ==Data== | ||
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| + | [https://houptlab.org/bartab/expt/?id=DML Raw Data Link] | ||
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| + | ==People== | ||
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| + | Durba Mukherjee | ||
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| + | ==CTA Conditioning== | ||
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| + | ===Agenda=== | ||
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| + | '''Rats arrive''' (August 10, 2021) | ||
| + | n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169. | ||
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| + | <br> | ||
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| + | '''Day 0: Water removed''' ( August 17, 2021) | ||
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| + | <br> | ||
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| + | '''Days 1 - 8: Water Restriction''' (August 18 - 25, 2021) | ||
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| + | Weigh rats daily. Water for 30 min, ad lib food. | ||
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| + | 30 min water at 1430 daily. | ||
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| + | <br> | ||
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| + | '''Day 9: Conditioning Day''' (August 26 2021) | ||
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| + | -15 min: All rats injected with 15mM Na Valproate 8ml/kg = 20mg/kg | ||
| + | 0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) | ||
| + | 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. | ||
| + | 40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip. | ||
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| + | ad lib water returned after final injection overnight. | ||
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| + | * rats 1-8 : female, Rats 9-16: male | ||
| + | * rats 1-16: Valproate; | ||
| + | * All rats: saccharin | ||
| + | * rats 1-4,9-12: 3ml/kg of 0.15M LiCl; rats 5-8,13-16: 3ml/kg of 0.15M NaCl | ||
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| + | '''Days 12 - ???: Preference Tests''' (August 27 - ??? , 2021) | ||
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| + | Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 12 days. | ||
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| + | [[Category:Flavor Preference]] | ||
| + | [[Category:Taste Aversion]] | ||
Latest revision as of 14:43, 26 August 2021
Repeat of DMJ Valproate and Saccharin CTA but with 20 mg/kg dose of valproate instead of 200 mg/kg dose.]]
Low Dose Valproate Dilution
5 ml of 0.15M Sodium Valproate + 45 ml of 0.15M NaCl (to maintain 300 mOsm tonicity)
--> 50 ml of 15mM Sodium Valproate (a tenfold dilution), at 2.5 mg/ml.
8 ml/kg = 20 mg/kg
Data
People
Durba Mukherjee
CTA Conditioning
Agenda
Rats arrive (August 10, 2021) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed ( August 17, 2021)
Days 1 - 8: Water Restriction (August 18 - 25, 2021)
Weigh rats daily. Water for 30 min, ad lib food.
30 min water at 1430 daily.
Day 9: Conditioning Day (August 26 2021)
-15 min: All rats injected with 15mM Na Valproate 8ml/kg = 20mg/kg 0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. 40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.
ad lib water returned after final injection overnight.
- rats 1-8 : female, Rats 9-16: male
- rats 1-16: Valproate;
- All rats: saccharin
- rats 1-4,9-12: 3ml/kg of 0.15M LiCl; rats 5-8,13-16: 3ml/kg of 0.15M NaCl
Days 12 - ???: Preference Tests (August 27 - ??? , 2021)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 12 days.