DMT Ovariectomy and Butyrate-Enhanced CTA 3

Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of DMP Ovariectomy and Butyrate-Enhanced CTA.

Ovariectomy and Butyrate-Enhanced CTA 3
HDAC Sex Difference
Expt Code	DMT
Start Date	2022-1-18
Investigators	Mukherjee, Houpt
Funding	Chair's Fund
Protocol	1917
Expt Type	CTA
Species	rat
Drugs	butyrate, lithium, estradiol
Data Link	https://houptlab.org/bartab/expt/?id=DMT

Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962

• 0.3 M Na Butyrate = 3.3g/100ml water
• 0.3 M NaCl = 18g/liter water (osmotic control)

Data

Raw Data Link

People

Durba Mukherjee

Agenda

Rats arrive Female Sprague Dawley rats n=8 (DMT1-5: dob 2021-9-21; rcvd 2021-12-7. DMT6-8: dob 2021-10-25;rcvd 2022-1-11

Surgery

all rats: ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)

• DMT 1-4 : Surgery on 2022-1-18
• DMT 5-8 : Surgery on 2022-1-19

Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.

Day 0: Water removed 2022-1-27

Days 1-8: Water Restriction 2022-1-28 thru 2022-2-4

Weigh rats daily. Water for 10 min, ad lib food.

10 min water at 1400 daily.

Day 9: Conditioning Day 2022-2-8

• 0-30 min: saccharin 0.125%

• at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)

• at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl

ad lib water returned after final injection overnight.

Groups:

• OVX + E + LiCl + NaCl: DMT 2,3,4,6
• OVX + E + LiCl + Butyrate: DMT 1,5,7,8

Days 10 - 19: Preference Tests: 2022-2-7 to 2022-2-16

Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.