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edited organization of background and agenda
Results suggest that selenate interferes with the regulation of learning-induced intracellular signaling by PP2A, and prevents consolidation of memories acquired during CTA learning. However, an alternative explanation is that sodium selenate affects LiCl-induced malaise and prevents the rats from associating the conditioned stimulus (saccharin taste) with LiCl by dampening the effects of LiCl toxicity.
Results suggest that selenate interferes with the regulation of learning-induced intracellular signaling by PP2A, and prevents consolidation of memories acquired during CTA learning. However, an alternative explanation is that sodium selenate affects LiCl-induced malaise and prevents the rats from associating the conditioned stimulus (saccharin taste) with LiCl by dampening the effects of LiCl toxicity.
Rats typically display lying-on-belly (LOB) behavior in response to LiCl-induced malaise. The time to onset of LOB behavior has been shown to decrease as the dose of LiCl increases (2), so the time to onset of LOB can be used to infer the magnitude of LiCl-induced malaise. This experiment aims to determine if sodium selenate pretreatment weakens the toxic effects of LiCl by measuring the time to onset of LOB after LiCl (0.15M, 20 ml/kg, i.p.) administration in selenate-pretreated vs control rats.
Rats typically display lying-on-belly (LOB) behavior in response to LiCl-induced malaise. The time to onset of LOB behavior has been shown to decrease as the dose of LiCl increases (2), so the time to onset of LOB can be used to infer the magnitude of LiCl-induced malaise.
The [[MBF Selenate Lying on Belly]] experiments determined that a 20 ml/kg i.p. injection of 0.15 LiCl reliably induces LOB behavior in male Sprague Dawley rats within 30 minutes.
The first set of LOB experiments (MBF01-06) determined that a 20 ml/kg i.p. injection of 0.15 LiCl reliably induces LOB behavior in male Sprague Dawley rats within 30 minutes.
*The low dose of LiCl (0.15M, 6 ml/kg, i.p.) did not induce LOB behavior
*The low dose of LiCl (0.15M, 6 ml/kg, i.p.) did not induce LOB behavior
*The moderate dose of LiCl (0.15M, 12 ml/kg, i.p.) reliably induced pica but did not induce LOB in all of the rats.
*The moderate dose of LiCl (0.15M, 12 ml/kg, i.p.) reliably induced pica but did not induce LOB in all of the rats.
This experiment aims to determine if sodium selenate pretreatment weakens the toxic effects of LiCl by measuring the time to onset of LOB after LiCl (0.15M, 20 ml/kg, i.p.) administration in selenate-pretreated vs control rats.
=Agenda=
MBG01-08
MBG01-08