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Line 35: − + − + − + − − 2. Isotonic LiCl and sesame oil, N=6 − − 3. Isotonic saline and 4-OHT, N=6 − 4. Isotonic saline and sesame oil, N=5+ − + Line 62:
Line 60: − + − + − + − * LVL: + Control (expect to form taste aversion)+ − * VTV: - Control (expect to not form taste aversion) − − + − + − + − +
JCF Excitatory DREADD Functional Validation (view source)
Revision as of 15:36, 27 February 2024
, 27 February→Agenda
CNO preparation (e.g. vehicle, how its dissolved)
CNO preparation (e.g. vehicle, how its dissolved)
4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c
{{Infobox experiment|align=right
{{Infobox experiment|align=right
| Name = Clozapine-N-Oxide Taste Aversion
| Name = Clozapine-N-Oxide Taste Aversion
| project = DREADDs and Magentic Field
| project = DREADDs and Magentic Field
| code = JCE
| code = JCF
| protocol = 202200000006
| protocol = 202200000006
| funding = Chair's Fund
| funding = Chair's Fund
| investigators = Cote, Houpt
| investigators = Cote, Houpt
| species = mice
| species = mice
| date = 2023-6-19
| date = 2024-1-28
| type = CTA
| type = CTA
| drugs = NaCl, CNO
| drugs = NaCl, CNO
==Agenda==
==Agenda==
Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)
Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Pre Treatment (8/18/23) 2 weeks before starting water restriction
Pre Treatment (1/28/24) 2 weeks before starting water restriction
4 groups:
4 groups:
1. Isotonic LiCl and 4-OHT, N=6
1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed
2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed
'''Day 0: Water removed''' (2023-9-12)
'''Day 0: Water removed''' (**2024-7-19)
In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.
In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.
'''Day 12: Conditioning Day''' (2023-9-24)
'''Day 12: Conditioning Day''' (2024-2-28)
Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min
Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min
Groups:
Groups:
* LTL: Exp group (predicted to show attenuated taste aversion)
* LTC: Exp group (Predicted to form taste aversion)
* VVC: Control (expect to not form taste aversion)
* VVV: - Control (expect to not form taste aversion)
* JCE01-04: Female
* JCF01-04: ???
* JCE05-08: Female
* JCF05-08: ???
* JCE09-12: Male
* JCF09-12: ???
* JCE13-16: Male
* JCF13-16: ???
After injection, ad lib water returned overnight.
After injection, ad lib water returned overnight.