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Test of role of testicular hormones in butyrate-enhanced CTA.
{{Infobox experiment|align=right
| Name = Orchiectomy and Butyrate-Enhanced CTA
| project = HDAC Sex Difference
| code = DMX
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Houpt, Sullivan
| species = rat
| date = 2024-5-20
| type = CTA
| drugs = butyrate, lithium
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=DMX
}}
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385)
* 0.3 M NaCl = 1.8g/100ml water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMX Raw Data Link]'''
==People==
Jessica Sullivan
==Agenda==
'''Rats arrive'''
Male Sprague Dawley rats n = 16, d.o.b. 2024-XX-XX, arrived: 2024-5-14
<br>
'''Surgery'''
* DMX 1-8: Orchiectomy Surgery on 2024-5-XX
* DMX 9-16: Sham Surgery on 2024-5-XX
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed 2024-XX-XX'''
<br>
'''Days 1-8: Water Restriction 2024-XX-XX thru 2024-XX-XX'''
Weigh rats daily. Ad lib food.
30 min, 30 min, 15 min, 10 min water at 1530-1600 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
<br>
'''Day 9: Conditioning Day 2024-XX-XX'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or 0.3M NaCl
ad lib water returned after final injection overnight.
Groups:
* ORC + LiCl + NaCl: DMX01-04
* ORC + LiCl + Butyrate: DMX05-08
* Sham + LiCl + NaCl: DMX09-12
* Sham + LiCl + Butyrate: DMX13-16
<br>
'''Days 10 - 19: Preference Tests: 2024-XX-XX to XXXX'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
[[Category:Epigenetics]]
{{Infobox experiment|align=right
| Name = Orchiectomy and Butyrate-Enhanced CTA
| project = HDAC Sex Difference
| code = DMX
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Houpt, Sullivan
| species = rat
| date = 2024-5-20
| type = CTA
| drugs = butyrate, lithium
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=DMX
}}
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385)
* 0.3 M NaCl = 1.8g/100ml water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMX Raw Data Link]'''
==People==
Jessica Sullivan
==Agenda==
'''Rats arrive'''
Male Sprague Dawley rats n = 16, d.o.b. 2024-XX-XX, arrived: 2024-5-14
<br>
'''Surgery'''
* DMX 1-8: Orchiectomy Surgery on 2024-5-XX
* DMX 9-16: Sham Surgery on 2024-5-XX
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed 2024-XX-XX'''
<br>
'''Days 1-8: Water Restriction 2024-XX-XX thru 2024-XX-XX'''
Weigh rats daily. Ad lib food.
30 min, 30 min, 15 min, 10 min water at 1530-1600 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
<br>
'''Day 9: Conditioning Day 2024-XX-XX'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or 0.3M NaCl
ad lib water returned after final injection overnight.
Groups:
* ORC + LiCl + NaCl: DMX01-04
* ORC + LiCl + Butyrate: DMX05-08
* Sham + LiCl + NaCl: DMX09-12
* Sham + LiCl + Butyrate: DMX13-16
<br>
'''Days 10 - 19: Preference Tests: 2024-XX-XX to XXXX'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
[[Category:Epigenetics]]