Difference between revisions of "JCG Inhibitory DREADD Functional Validation"

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(Created page with "==Agenda== Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-) Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at...")
 
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To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion.
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CNO ordering information / lot numbers
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CNO preparation (e.g. vehicle, how its dissolved)
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4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c
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{{Infobox experiment|align=right
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| Name = Clozapine-N-Oxide Taste Aversion
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| project = DREADDs and Magentic Field
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| code = JCF
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| protocol = 202200000006
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| funding = Chair's Fund
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| investigators = Cote, Houpt
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| species = mice
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| date = 2024-1-28
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| type = CTA
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| drugs = NaCl, CNO
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| artifacts =
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| data = https://houptlab.org/bartab/expt/?id=JCF}}
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==Data==
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'''[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]'''
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==People==
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Jason Cote
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==Agenda==
 
==Agenda==
  

Revision as of 12:58, 31 May 2024

To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be reactivated with CNO administration to induce a taste aversion.


CNO ordering information / lot numbers

CNO preparation (e.g. vehicle, how its dissolved)

4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c

Clozapine-N-Oxide Taste Aversion
DREADDs and Magentic Field
Expt CodeJCF
Start Date2024-1-28
InvestigatorsCote, Houpt
FundingChair's Fund
Protocol202200000006
Expt TypeCTA
Speciesmice
DrugsNaCl, CNO
Data Linkhttps://houptlab.org/bartab/expt/?id=JCF


Data

Raw Data Link

People

Jason Cote

Agenda

Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)

Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.

Pre Treatment (1/28/24) 2 weeks before starting water restriction 4 groups:

1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed

2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed


Day 0: Water removed (**2024-7-19)


Days 1 - 11: Water Restriction (2023-9-13 - 2023-9-23)

Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.


Mice given water bottle and empty bottle, alternating position.

In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.

Day 12: Conditioning Day (2024-2-28)

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min

Groups:

  • LTC: Exp group (Predicted to form taste aversion)
  • VVC: Control (expect to not form taste aversion)


  • JCG01-05: ???
  • JCG06-10: ???


After injection, ad lib water returned overnight.

Day 13 - 24: Preference Tests (2023-9-25 - 2023-10-6 )

CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.

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