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Follow Enzo R18 perm redirect
==14-3-3==
==14-3-3==
https://ghr.nlm.nih.gov/gene/YWHAE
https://medlineplus.gov/genetics/gene/ywhae/
Ywhae gene = 14-3-3 epsilon = tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae)
Ywhae gene = 14-3-3 epsilon = tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae)
Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA
Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA
NCBI Reference Sequence: NM_009536.4
NCBI Reference Sequence: NM_009536.4
==Dr. Yi Zhou's 14-3-3 FKO (Thy1-YFP-DIfopein) Transgenic Mice==
==Dr. Yi Zhou's 14-3-3 FKO (Thy1-YFP-DIfopein) Transgenic Mice==
PMID 11577088
PMID 11577088
R18 is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6) which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [Enzo #BML-P214-0001 https://www.enzolifesciences.com/BML-P214/r18-peptide/].
R18 is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6) which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [https://www.enzo.com/product/r18-peptide/ Enzo #BML-P214-0001].
Difopein is a construct with two R18 monomers in a single 62 amino acid peptide PMID 11577088. Commercial source of peptide: [Tocris #1245 https://www.tocris.com/products/difopein_2145]
Difopein is a construct with two R18 monomers in a single 62 amino acid peptide PMID 11577088. Commercial source of peptide: [https://www.tocris.com/products/difopein_2145 Tocris #1245]
==YFP==
==YFP==
YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.thermofisher.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp).
YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [https://www.thermofisher.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp).
== 14-3-3 FKO papers==
== 14-3-3 FKO papers==
Vogt, Peggy, [http://diginole.lib.fsu.edu/islandora/object/fsu:204445 "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.
Vogt, Peggy, [https://diginole.lib.fsu.edu/islandora/object/fsu:204445 "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.
C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400
C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400