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DMX Orchiectomy and Butyrate-Enhanced CTA (view source)
Revision as of 13:10, 12 October 2024
, 13:10, 12 October 2024→Agenda: updated restriction time
| protocol = 202200000006
| protocol = 202200000006
| funding = Chair's Fund
| funding = Chair's Fund
| investigators = Houpt, Sullivan
| investigators = Mukeherjee, Bass, Houpt
| species = rat
| species = rat
| date = 2024-5-20
| date = 2024-6-19
| type = CTA
| type = CTA
| drugs = butyrate, lithium
| drugs = butyrate, lithium
}}
}}
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.15 M LiCl = 3.18g / 500ml water
* 0.15 M LiCl = 3.18g / 500ml water
==People==
==People==
Houpt
Mukherjee, Bass, Houpt
==Agenda==
==Agenda==
* DMX 9-15: Sham Surgery on 2024-6-21
* DMX 9-15: Sham Surgery on 2024-6-21
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op. Sham rats received incision and staples.
Orchiectomy as per Sophocles and Idris 2019 PMID 30729469
Sham rats received incision and staples.
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
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'''Days 1-8: Water Restriction 2024-7-15 thru 2024-7-21'''
'''Days 1-6: Water Restriction 2024-7-15 thru 2024-7-20'''
Weigh rats daily. Ad lib food.
Weigh rats daily. Ad lib food.
30 min, 30 min, 15 min, 10 min water at 1530-1600 daily.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1415 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
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'''Day 9: Conditioning Day 2024-XX-XX'''
'''Day 7: Conditioning Day 2024-7-21'''
* 0-30 min: saccharin 0.125%
* 0-30 min: saccharin 0.125%
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'''Days 10 - 19: Preference Tests: 2024-XX-XX to XXXX'''
'''Days 8 - ???: Preference Tests: 2024-7-22 to 2024-8-1'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 10 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.