Difference between revisions of "GFA phospho-PDH and Taste Aversion"
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Revision as of 12:34, 7 December 2024
Examine levels of phosphorylated pyruvate dehydrogenase by immunofluorescence in after intraoral conditioned taste aversion.
Based on Yang et al. 2024 PMID 38266644 and report by Roitman lab at SSIB 2024.
Employed SD-GFP-TH rats (Iacovitti et al 2014 PMID: 25462571) from Taconic that express GFP under TH promoter with especially good expression in VTA (Iacovitti et al 2014 PMID 25462571).
| phospho-PDH and CTA pt1 | |
|---|---|
| Taste Aversion | |
| Expt Code | GFA |
| Start Date | 2024-12-3 |
| Investigators | Houpt, Cote, Trombley, Blakemore |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=GFA |
Data
People
Jason Cote (breeding), Laura Blakemore and Paul Trombley (colony foundation and management)
CTA Conditioning
Agenda
Rats Arrive (December 3, 2024)
Rats (n=4 male, n=5 female, littermates, DOB: 7-???-2024) transferred from GFP-TH breeding protocol to individual cages.
Day 0: Surgery (December XXX, 2024)
Chronic Intraoral Catheters implanted under isoflurane anesthesia. All rats receive carprofen (XXX mg/kg) pre-operatively and 1 day post-op.
Days 1 - 3: Recovery (December XXXX - XXXX, 2024)
Weigh rats daily. Ad lib food and water. Carprofen (XXX mg/kg) on first day post-op.
Days 5-7: Habituation (December XXXX - XXXX, 2024)
Rats given intraoral infusion of 6 ml dH2O over 6 min to habituate to infusion procedure
Food removed on last day of habituation.
Noncontingent group: Half of rats injected with LiCl (0.15M, 12 ml/kg).
Day 8: Conditioning day 1 (December XXXX, 2024)
All rats given intraoral infusion of 6 ml sucrose 5% over 6 min.
Contingent group: Half of rats injected with LiCl (0.15M, 12 ml/kg).
Food returned overnight.
Day 9: Recovery Day (December XXXX, 2024)
Food removed.
Noncontingent group: Half of rats injected with LiCl (0.15M, 12 ml/kg).
Day 10: Conditioning day 2 (December XXXX, 2024)
All rats given intraoral infusion of 6 ml sucrose 5% over 6 min.
Contingent group: Half of rats injected with LiCl (0.15M, 12 ml/kg).
Food returned overnight.
Day 11: Recovery Day (December XXXX, 2024)
Food removed.
Noncontingent group: Half of rats injected with LiCl (0.15M, 12 ml/kg).
Day 12: Conditioning day 2 (December XXXX, 2024)
All rats given intraoral infusion of 6 ml sucrose 5% over 6 min.
Contingent group: Half of rats injected with LiCl (0.15M, 12 ml/kg).
Food returned overnight.
Day 13: Recovery Day (December XXXX, 2024)
Food removed.
Day 14: Expression Test (December XXXX, 2024)
All rats given intraoral infusion of 6 ml sucrose 5% over 6 min.
Ten minutes after infusion, rats are overdosed with euthansol and transcardially perfused. Brains postfixed for 1-2 hours, then stored in 0.1M PB.