Difference between revisions of "DMW Ovariectomy and Butyrate-Enhanced CTA 4"
(added water restriction) |
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| protocol = 202200000006 | | protocol = 202200000006 | ||
| funding = Chair's Fund | | funding = Chair's Fund | ||
| − | | investigators = Houpt, Bass | + | | investigators = Houpt, Bass, D. Jorge |
| species = rat | | species = rat | ||
| date = 2023-6-18 | | date = 2023-6-18 | ||
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Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] | Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] | ||
| − | * 0.3 M Na Butyrate = 3.3g/100ml water | + | * 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385) |
| − | * 0.3 M NaCl = | + | * 0.3 M NaCl = 1.8g/100ml water (osmotic control) |
<br> | <br> | ||
| Line 50: | Line 50: | ||
| − | '''Day 0: Water removed 2023-7-2'' | + | '''Day 0: Water removed 2023-7-2''' |
<br> | <br> | ||
| − | '''Days 1-8: Water Restriction 2023-7-3 thru | + | '''Days 1-8: Water Restriction 2023-7-3 thru 2023-7-10''' |
Weigh rats daily. Water for 10 min, ad lib food. | Weigh rats daily. Water for 10 min, ad lib food. | ||
| − | 30 min, 30 min, then | + | 30 min, 30 min, then 15 min water at 1530-1600 daily. |
<br> | <br> | ||
| − | '''Day 9: Conditioning Day | + | '''Day 9: Conditioning Day 2023-7-11''' |
* 0-30 min: saccharin 0.125% | * 0-30 min: saccharin 0.125% | ||
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* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats) | * at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats) | ||
| − | * at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl | + | * at 40 min: 12 ml/kg of 0.3M NaButyrate or 0.3M NaCl |
ad lib water returned after final injection overnight. | ad lib water returned after final injection overnight. | ||
| Line 75: | Line 75: | ||
| − | * OVX + LiCl + NaCl: | + | * OVX + LiCl + NaCl: DMW02, 03, 04 |
| − | * OVX + LiCl + Butyrate: DMW | + | * OVX + LiCl + Butyrate: DMW 05, 06, 12 |
| − | * Sham + LiCl + NaCl: DMW | + | * Sham + LiCl + NaCl: DMW 07, 08, 09 |
| − | * Sham + LiCl + Butyrate: DMW | + | * Sham + LiCl + Butyrate: DMW 10, 11 |
<br> | <br> | ||
| − | '''Days 10 - 19: Preference Tests: | + | '''Days 10 - 19: Preference Tests: 2023-7-12 to XXXX''' |
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days. | Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days. | ||
Latest revision as of 14:25, 11 July 2023
Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of DMP Ovariectomy and Butyrate-Enhanced CTA.
| Ovariectomy and Butyrate-Enhanced CTA 4 | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMW |
| Start Date | 2023-6-18 |
| Investigators | Houpt, Bass, D. Jorge |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | butyrate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMW |
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385)
- 0.3 M NaCl = 1.8g/100ml water (osmotic control)
Data
People
Marena Bass, Daniela Jorge, Durba Mukherjee
Agenda
Rats arrive Female Sprague Dawley rats n = 12, d.o.b. 2023-12-2, arrived: 2023-2-21
Surgery
- DMW 1-6: Ovariectomy Surgery on 2023-6-18
- DMW 7-11: Sham Surgery on 2023-6-24
- DMW 12: Ovariectomy Surgery on 2023-6-24
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
Day 0: Water removed 2023-7-2
Days 1-8: Water Restriction 2023-7-3 thru 2023-7-10
Weigh rats daily. Water for 10 min, ad lib food.
30 min, 30 min, then 15 min water at 1530-1600 daily.
Day 9: Conditioning Day 2023-7-11
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
- at 40 min: 12 ml/kg of 0.3M NaButyrate or 0.3M NaCl
ad lib water returned after final injection overnight.
Groups:
- OVX + LiCl + NaCl: DMW02, 03, 04
- OVX + LiCl + Butyrate: DMW 05, 06, 12
- Sham + LiCl + NaCl: DMW 07, 08, 09
- Sham + LiCl + Butyrate: DMW 10, 11
Days 10 - 19: Preference Tests: 2023-7-12 to XXXX
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.