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Line 32:
Line 32: − reuse same rats, shuffled into 4 groups: NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl+ − at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)+ − at 0 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)+ − + + + + + + + + + + +
revised c-fos experiment
'''2. Test c-Fos induction'''
'''2. Test c-Fos induction'''
2012-1-10 perfused JB 1-5
2012-3-14 perfuse JB 6-10
Reuse same rats, shuffled into new groups:
at 1 h, perfuse rats
at -2 h, Inject with sodium selenate in NaCl 0.15 M , 1 ml/kg at 4 doses:
*0 mg/kg Na2Se04
*0.5 mg/kg Na2Se04
*1 mg/kg Na2Se04
*2 mg/kg Na2Se04
and 0.15M LiCl 12 ml/kg as a positive control.
at 0 h, perfuse rats.
process for c-Fos (~ 1 week in the lab)
process for c-Fos (~ 1 week in the lab)
[[Category: CTA Experiments]]
[[Category: CTA Experiments]]