Difference between revisions of "Oligo Tail Labeling"
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[ format as for cDNA Probe Labeling] | [ format as for cDNA Probe Labeling] | ||
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Latest revision as of 11:51, 5 February 2013
For labeling DNA oligonucleotide probes for use for In Situ Hybridization.
Reagents:
Terminal Transferase Labeling Kit,
dATP-alphaS35, (250 uCi vial = 20 µl) Perkin Elmer #NEG034H250UC $172
- made first Tuesday of every month
ProbeQuant G-50 micro spin columns, 50 pack, GE Healthcare, # 28-9034-08 $199
Procedure:
Take radioactivity out to melt (1 h if isotope in thick glass vial).
Turn on heat block to 100° C.
[ format as for cDNA Probe Labeling]