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Anorexia anx/anx mutant mice
(view source)
Revision as of 15:38, 6 June 2024
21 bytes added
,
6 June
typo
Line 15:
Line 15:
== Tyro3 Genotyping ==
== Tyro3 Genotyping ==
−
TYRO3 protein tyrosine kinase 3 (Tyro3) mutation in anx/anx
eliminatees
a NlaIV restriction site.
+
TYRO3 protein tyrosine kinase 3 (Tyro3) mutation
(C19T-Tyro3 mutation)
in anx/anx
eliminates
a NlaIV restriction site.
* primers forward 5′-GATGGCGCTGAGGCGGAGCATG and reverse 5′-CGCGGCCGGAGGTCTGGCAG ; annealing temperature 72C
* primers forward 5′-GATGGCGCTGAGGCGGAGCATG and reverse 5′-CGCGGCCGGAGGTCTGGCAG ; annealing temperature 72C
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