Difference between revisions of "Serotonin Genes"

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I am interested in 4 of the genes related to serotonin function in depression and food intake:
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I am interested in 4 of the genes related to serotonin function in depression and food intake: Pet-1, Serotonin Transporter (5HTT), Tryptophan Hydroxylase 2 (TPH2), and 5HT2C Receptor (5HT2CR)
  
Pet-1
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= ISH probes=
  
Serotonin Transporter
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==Pet-1==
  
Tryptophan Hydroxylase 2
 
  
5HT2C Receptor
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Reference:
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==Serotonin Transporter==
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Reference:
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 +
 
 +
==Tryptophan Hydroxylase 2==
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 +
 
 +
(see PCR primers below).
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 +
 
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Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992
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==5HT2C Receptor==
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(see PCR primers below)
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Reference:
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Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992
 
Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992
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==Tryptophan Hydroxylase 2 polymorphisms==
 
==Tryptophan Hydroxylase 2 polymorphisms==
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Reference:  Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473
 
Reference:  Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473
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==5HT2C Receptor cDNA==
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for in situ hybridization
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product size:  bp, range & ascension number?
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:5HT2Cfor: 5’ -CTATCCCTTACCTTCCTATTAC–3’
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:5HT2Crev: 5’ -TACCCTACTCTAGCATAGAAAC–3’
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Reference: ???

Revision as of 13:04, 24 September 2007

I am interested in 4 of the genes related to serotonin function in depression and food intake: Pet-1, Serotonin Transporter (5HTT), Tryptophan Hydroxylase 2 (TPH2), and 5HT2C Receptor (5HT2CR)

ISH probes

Pet-1

Reference:

Serotonin Transporter

Reference:


Tryptophan Hydroxylase 2

(see PCR primers below).


Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992

5HT2C Receptor

(see PCR primers below)

Reference:


PCR primers

Tryptophan Hydroxylase 2 cDNA

for in situ hybridization

product size: bp, range & ascension number?

TPH2for: 5’ -GAAGTTGGCGGGCTGGTGAGA–3’
TPH2rev: 5’ -TCGGCAAGCATGAGTGGGGTAGA–3’

Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992


Tryptophan Hydroxylase 2 polymorphisms

linked to aggression. 5 min at 94°C then 40 cycles (30 s at 94°C, 30 s at 60°C, 30 s at 72°C)

Positive control, product size 523 bp

ctrlTPH2for: 5’ -tttgacccaaagacgacctgcttgca –3’
ctrlTPH2rev: 5’-tgcatgcttactagccaaccatgagaca-3’
1473C allele, product size 307bp

TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’

1473G allele. product size 307 bp

TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’

Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473

5HT2C Receptor cDNA

for in situ hybridization

product size: bp, range & ascension number?

5HT2Cfor: 5’ -CTATCCCTTACCTTCCTATTAC–3’
5HT2Crev: 5’ -TACCCTACTCTAGCATAGAAAC–3’

Reference: ???