Difference between revisions of "C-Fos Methylation"
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==DNA Methylation sites in c-Fos promoter== | ==DNA Methylation sites in c-Fos promoter== | ||
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| + | [[Image:c-FosCpGs.jpg|thumb|none|300px|From Shimizu et al., Cancer Sci 2007; 98: 1318–1322]] | ||
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Approximate MW: | Approximate MW: | ||
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each methylation site causes 15 dalton decrease in MW | each methylation site causes 15 dalton decrease in MW | ||
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| + | ==Rat c-fos Primers== | ||
From Shimizu et al., Cancer Sci 2007; 98: 1318–1322 | From Shimizu et al., Cancer Sci 2007; 98: 1318–1322 | ||
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'''Bisulfite sequencing (Genomic DNA)''' | '''Bisulfite sequencing (Genomic DNA)''' | ||
Revision as of 08:57, 24 April 2008
DNA Methylation sites in c-Fos promoter
Approximate MW:
38,337 daltons (unmethylated)
38,252 daltons (methylated)
each methylation site causes 15 dalton decrease in MW 
Rat c-fos Primers
From Shimizu et al., Cancer Sci 2007; 98: 1318–1322
Bisulfite sequencing (Genomic DNA)
GenBank AF126534
F: 5′-TATTTATAGGTGAAAGTTATAGATTG-3′
R: 5′-CACTAATAAAAACTACAAAACAAAACT-3′
Annealing temperature 54
Product size 292 bases
4-23-08 -- could NOT confirm with blast -- need to check earlier references
RT-PCR (from RNA)
GenBank X06769
F: 5′-TTGCGCAGATCTGTCCGTCT-3′
R: 5′-GTTGATCTGTCTCCGCTTGG-3′
Annealing temperature 65
4-23-08 -- confirmed with blast