Changes

688 bytes added ,  11:23, 28 April 2008
→‎5HT2C Receptor cDNA: added refs & ascension #
Line 74: Line 74:  
for in situ hybridization
 
for in situ hybridization
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product size:  bp, range & ascension number?
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product size: 207 bp, ascension number: M21410, bp 105-311
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:5HT2Cfor: 5’ -CTATCCCTTACCTTCCTATTAC–3’  
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:5HT2Cfor (bp 105-126): 5’ -CTATCCCTTACCTTCCTATTAC–3’  
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:5HT2Crev: 5’ -TACCCTACTCTAGCATAGAAAC–3’  
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:5HT2Crev (bp 311-290); 5’ -TACCCTACTCTAGCATAGAAAC–3’  
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Reference: ??? Still haven't found it
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References:  
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Heisler LK, Pronchuk N, Nonogaki K, Zhou L, Raber J, Tung L, Yeo GS, O'Rahilly S, Colmers WF, Elmquist JK, Tecott LH.
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Serotonin activates the hypothalamic-pituitary-adrenal axis via serotonin 2C receptor stimulation.
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J Neurosci. 2007 Jun 27;27(26):6956-64.
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PMID: 17596444
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Molineaux SM, Jessell TM, Axel R, Julius D.
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5-HT1c receptor is a prominent serotonin receptor subtype in the central nervous system.
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Proc Natl Acad Sci U S A. 1989 Sep;86(17):6793-7.
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PMID: 2771958
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Julius,D., MacDermott,A.B., Axel,R. and Jessell,T.M. Molecular characterization of a functional cDNA encoding the
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            serotonin 1c receptor, Science 241 (4865), 558-564 (1988) PMID: 3399891