Difference between revisions of "ICV Cannulation"
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(added dental acrylic) |
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| − | + | See also [[Amygdala]] for other cannulation coordinates. | |
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| + | ==Lateral Ventricular Cannula:== | ||
1.2 mm caudal to bregma | 1.2 mm caudal to bregma | ||
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4 mm below the skull surface | 4 mm below the skull surface | ||
| − | + | ===Method=== | |
Each rat was stereotaxically implanted with a 22 gauge, stainless steel, guide | Each rat was stereotaxically implanted with a 22 gauge, stainless steel, guide | ||
| Line 21: | Line 23: | ||
and replaced with an injector that extended 1.0 mm | and replaced with an injector that extended 1.0 mm | ||
beyond the tip of the guide cannula. | beyond the tip of the guide cannula. | ||
| + | |||
| + | ==Supplies== | ||
| + | |||
| + | New Truliner Denture Lining Standard Kit, Pink [http://www.bosworth.com/product_detail.asp?PRD_ID=18 Bosworth #0921970] $45.00 | ||
| + | |||
| + | :Harry J. Bosworth Company | ||
| + | :7227 North Hamlin Avenue | ||
| + | :Skokie, Illinois 60076 USA | ||
| + | :Toll-Free: 800-323-4352 | ||
| + | :Phone: 847-679-3400 | ||
| + | :Fax: 847-679-2080 | ||
| + | :E-Mail: HJBinfo@bosworth.com | ||
| + | |||
[[Category:Taste_Aversion]] | [[Category:Taste_Aversion]] | ||
Revision as of 10:38, 13 May 2011
See also Amygdala for other cannulation coordinates.
Lateral Ventricular Cannula:
1.2 mm caudal to bregma
1.5 mm lateral to the midline
4 mm below the skull surface
Method
Each rat was stereotaxically implanted with a 22 gauge, stainless steel, guide cannula (Plastics One, Roanoke, VA) aimed towards the lateral cerebral ventricle (1.2 mm caudal to bregma, 1.5 mm lateral to the midline, and 4 mm below the skull surface). Guide cannulas were held in place with dental acrylic bonded to stainless steel screws anchored to the skull. An obdurator was inserted into each guide cannula and remained in place except during injections when it was removed and replaced with an injector that extended 1.0 mm beyond the tip of the guide cannula.
Supplies
New Truliner Denture Lining Standard Kit, Pink Bosworth #0921970 $45.00
- Harry J. Bosworth Company
- 7227 North Hamlin Avenue
- Skokie, Illinois 60076 USA
- Toll-Free: 800-323-4352
- Phone: 847-679-3400
- Fax: 847-679-2080
- E-Mail: HJBinfo@bosworth.com