Difference between revisions of "DMP Ovariectomy and Butyrate-Enhanced CTA"
(added infobox) |
m |
||
| Line 1: | Line 1: | ||
| + | |||
| + | Test of role of ovarian hormones in butyrate-enhanced CTA. | ||
| + | |||
{{Infobox experiment|align=right | {{Infobox experiment|align=right | ||
| Name = Ovariectomy and Butyrate-Enhanced CTA pt1 | | Name = Ovariectomy and Butyrate-Enhanced CTA pt1 | ||
| Line 13: | Line 16: | ||
| data = https://houptlab.org/bartab/expt/?id=DMP | | data = https://houptlab.org/bartab/expt/?id=DMP | ||
}} | }} | ||
| − | |||
| − | |||
| − | |||
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] | Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] | ||
Revision as of 09:48, 5 July 2022
Test of role of ovarian hormones in butyrate-enhanced CTA.
| Ovariectomy and Butyrate-Enhanced CTA pt1 | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMP |
| Start Date | 2021-8-24 |
| Investigators | Mukherjee, Houpt |
| Funding | Chair's Fund |
| Protocol | 1917 |
| Expt Type | CTA |
| Species | rat |
| Drugs | butyrate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMP |
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Data
People
Durba Mukherjee
Agenda
Rats arrive (2021-08-24) Female Sprague Dawley rats n=18 (dob 2021-06-07), arrived
Surgery
3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
- DMP1-9: Surgery on 2021-10-24
- DMP10-18: Surgery on 2021-10-30
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
Day 0: Water removed 2021-11-08
Days 1-7: Water Restriction 2021-11-9 thru 2021-11-15
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
Day 8: Conditioning Day 2021-11-16
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
- at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
Groups:
- Sham + NaCl: DMP08, DMP17
- Sham + Butyrate: DMP07, DMP09, DMP16
- OVX + NaCl: DMP01, DMP10, DMP12
- OVX + Butyrate: DMP03,DMP11, DMP13
- OVX + E + NaCl: DMP04, DMP06
- OVX + E + Butyrate: DMP05, DMP14
ad lib water returned after final injection overnight.
Days 9 - 23: Preference Tests: 2021-11-17 to 2021-11-27
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 14 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.