DMX Orchiectomy and Butyrate-Enhanced CTA

Test of role of testicular hormones in butyrate-enhanced CTA.

Orchiectomy and Butyrate-Enhanced CTA
HDAC Sex Difference
Expt Code	DMX
Start Date	2024-5-20
Investigators	Houpt, Sullivan
Funding	Chair's Fund
Protocol	202200000006
Expt Type	CTA
Species	rat
Drugs	butyrate, lithium
Data Link	https://houptlab.org/bartab/expt/?id=DMX

Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962

• 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385)
• 0.3 M NaCl = 1.8g/100ml water (osmotic control)

Data

Raw Data Link

People

Jessica Sullivan

Agenda

Rats arrive Male Sprague Dawley rats n = 16, d.o.b. 2024-XX-XX, arrived: 2024-5-14

Surgery

• DMX 1-8: Orchiectomy Surgery on 2024-6-19
• DMX 9-16: Sham Surgery on 2024-5-XX

Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.

Day 0: Water removed 2024-XX-XX

Days 1-8: Water Restriction 2024-XX-XX thru 2024-XX-XX

Weigh rats daily. Ad lib food.

30 min, 30 min, 15 min, 10 min water at 1530-1600 daily.

1 water bottle, 1 empty bottle to accustom to 2-bottle choice

Day 9: Conditioning Day 2024-XX-XX

• 0-30 min: saccharin 0.125%

• at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)

• at 40 min: 12 ml/kg of 0.3M NaButyrate or 0.3M NaCl

ad lib water returned after final injection overnight.

Groups:

• ORC + LiCl + NaCl: DMX01-04
• ORC + LiCl + Butyrate: DMX05-08
• Sham + LiCl + NaCl: DMX09-12
• Sham + LiCl + Butyrate: DMX13-16

Days 10 - 19: Preference Tests: 2024-XX-XX to XXXX

Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.