Difference between revisions of "DMX Orchiectomy and Butyrate-Enhanced CTA"
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'''Rats arrive''' | '''Rats arrive''' | ||
| − | Male Sprague Dawley rats n = | + | Male Sprague Dawley rats n = 15, d.o.b. 2024-3-18, arrived: 2024-5-14 |
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* DMX 1-8: Orchiectomy Surgery on 2024-6-19 | * DMX 1-8: Orchiectomy Surgery on 2024-6-19 | ||
| − | * DMX 9- | + | * DMX 9-15: Sham Surgery on 2024-6-21 |
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op. Sham rats received incision and staples. | Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op. Sham rats received incision and staples. | ||
| − | '''Day 0: Water removed 2024- | + | '''Day 0: Water removed 2024-7-14''' |
<br> | <br> | ||
| − | '''Days 1-8: Water Restriction 2024- | + | '''Days 1-8: Water Restriction 2024-7-15 thru 2024-7-21''' |
Weigh rats daily. Ad lib food. | Weigh rats daily. Ad lib food. | ||
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* ORC + LiCl + Butyrate: DMX05-08 | * ORC + LiCl + Butyrate: DMX05-08 | ||
* Sham + LiCl + NaCl: DMX09-12 | * Sham + LiCl + NaCl: DMX09-12 | ||
| − | * Sham + LiCl + Butyrate: DMX13- | + | * Sham + LiCl + Butyrate: DMX13-15 |
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Revision as of 13:11, 14 July 2024
Test of role of testicular hormones in butyrate-enhanced CTA.
| Orchiectomy and Butyrate-Enhanced CTA | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMX |
| Start Date | 2024-5-20 |
| Investigators | Houpt, Sullivan |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | butyrate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMX |
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385)
- 0.3 M NaCl = 1.8g/100ml water (osmotic control)
Data
People
Jessica Sullivan
Agenda
Rats arrive Male Sprague Dawley rats n = 15, d.o.b. 2024-3-18, arrived: 2024-5-14
Surgery
- DMX 1-8: Orchiectomy Surgery on 2024-6-19
- DMX 9-15: Sham Surgery on 2024-6-21
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op. Sham rats received incision and staples.
Day 0: Water removed 2024-7-14
Days 1-8: Water Restriction 2024-7-15 thru 2024-7-21
Weigh rats daily. Ad lib food.
30 min, 30 min, 15 min, 10 min water at 1530-1600 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
Day 9: Conditioning Day 2024-XX-XX
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
- at 40 min: 12 ml/kg of 0.3M NaButyrate or 0.3M NaCl
ad lib water returned after final injection overnight.
Groups:
- ORC + LiCl + NaCl: DMX01-04
- ORC + LiCl + Butyrate: DMX05-08
- Sham + LiCl + NaCl: DMX09-12
- Sham + LiCl + Butyrate: DMX13-15
Days 10 - 19: Preference Tests: 2024-XX-XX to XXXX
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.