DMR Ovariectomy and Butyrate-Enhanced CTA 2

Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of DMP Ovariectomy and Butyrate-Enhanced CTA.

NOTE: concerned about low bodyweight of rats, amd that butyrate was not freshly made.

Ovariectomy and Butyrate-Enhanced CTA 2
HDAC Sex Difference
Expt Code	DMR
Start Date	2022-1-9
Investigators	Mukherjee, Houpt
Funding	Chair's Fund
Protocol	1917
Expt Type	CTA
Species	rat
Drugs	butyrate, lithium, estradiol
Data Link	https://houptlab.org/bartab/expt/?id=DMR

Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962

• 0.3 M Na Butyrate = 3.3g/100ml water
• 0.3 M NaCl = 18g/liter water (osmotic control)

Data

Raw Data Link

People

Durba Mukherjee

Agenda

Rats arrive (Charles River 2021-12-7) Female Sprague Dawley rats n=13 (dob 2021-9-20

Surgery

3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)

• DMR 01-06: Surgery on 2021-12-30
• DMR 07-12: Surgery on 2021-12-31
• DMR 13: Surgery on 2022-1-2

Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.

Day 0: Water removed 2022-1-09

Days 1-7: Water Restriction 2022-1-9 thru 2022-1-17

Weigh rats daily. Water for 10 min, ad lib food.

10 min water at 1400 daily.

Day 8: Conditioning Day 2022-1-18

• 0-30 min: saccharin 0.125%

• at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)

• at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl

ad lib water returned after final injection overnight.

Groups:

• Sham + NaCl: DMR04,DMR06,DMR10,DMR12
• Sham + Butyrate: DMR05,DMR11,DMR13
• OVX + NaCl: DMR01, DMR03,DMR08
• OVX + Butyrate: DMR02,FMR07,DMR09

Days 9 - 23: Preference Tests: 2022-1-19 to ????

Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.