C-Fos-Cre DREADD

Overview

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  Chemogenetic expression of DREADD receptors in c-Fos-positive cells after stimulation

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  Schematic timeline of recapitulating stimulation with excitatory DREADDs

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  Schematic timeline of attenuating stimulation with inhibitory DREADDs

Mouse Strains

Fos-CreER

Fos-tm2.1(icre/ERT2)Luo (JAX 030323)

hM3Dq

B6N;129-Tg(CAG-CHRM3*,-mCitrine)1Ute/J, aka CAG-LSL-Gq-DREADD (JAX 026220)

hM4Di

B6N.129-Gt(ROSA)26Sortm1(CAG-CHRM4*,-mCitrine)Ute/J, aka R26-LSL-Gi-DREADD (JAX 026219)

Reagents

(Z)-4-Hydroxytamoxifen , Sigma-Aldrich H7904-5MG

Clozapine N-oxide (CNO) . APExBIO A3317 10mg

DMSO USP, Spectrum Chemical D1258-100ML

Sesame Oil, NF Spectrum Chemical SE130-500MLGL