Vestibular Mutant Mice

Phenotyping

A swim test was used to phenotype het mice, tlt mice, and their littermates. Wildtype mice (+/+) swam toward the side of the pool. Homozygous het mice (het/het) and tlt mice (tlt/tlt) were identified by their inability to swim while keeping their head above water; instead, they swam in circles and somersault towards underwater. Heterozygotes (het/+ or tlt/+) were identified by an intermediate phenotype.

het

spontaneous mutation B6 x STOCK Nox3^het/J, Common name: head-tilt

Strain #000061

PCR protocol

tlt

B6.Cg-Otop1^tlt/J, Common Name: tilted

Strain #001104

Sequencing protocol