DMP Ovariectomy and Butyrate-Enhanced CTA

Test of role of ovarian hormones in butyrate-enhanced CTA.

3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)

DMP1-9: Surgery on 2021-10-24 DMP10-18: Surgery on 2021-10-30

Female Sprague Dawley rats (dob 2021-06-07), arrived 2021-08-24.

Water removed 2021-11-08

Restriction 2021-11-9 thru 2021-11-15

Conditioning Day 2021-11-16

• 0-30 min: saccharin 0.125%

• at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)

• at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl

Water returned.

Two-Bottle Test Days: 2021-11-17 to ???