NOB Novel Object Recognition Pilot
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Pilot to test Novel Object Recognition (NOR) set-up.
(describe arena setup)
| NOB Novel Object Recognition Pilot | |
|---|---|
| Memory | |
| Expt Code | SNB |
| Start Date | 2026-6-22 |
| Investigators | Abreu-Price, Houpt |
| Funding | Chair's Fund |
| Protocol | 20220000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | NA |
| Data Link | NA |
People
Julia Abreu-Price
Agenda
NOB01-06, Adult male Sprague Dawley rats (Charles River), DOB 2026-4-12, arrived 2026-6-9, housed in BRF169.
All arena tests run in BRF115.
1-hour Retention Test
Habituation phase (2026-6-22,23,24)
For each rat, once per day for 3 consecutive days:
- wipe down sides of arena with Windex multisurface disinfectant cleaner
- place new wrap under arena
- bring rat cage from housing rm BRF169 to test room BRF115, weigh rat, place in arena for 3 minutes, return to home cage.
Training (2026-6-25)
- wipe down sides of arena with Windex multisurface disinfectant cleaner
- place new wrap under arena
- place 2 identical toys (i.e., 2 x kong or 2 x jack) in corners 20cm away from each wall
- bring rat cage from housing rm BRF169, weigh rat
- start video recording on iMac, place rat in arena for 3 (THREE) minutes
- return to home cage and BRF 169.
- wash toys in soapy water and rinse
- repeat for each rat, alternating toys:
* 2 x Kong: NOB01,NOB03,NOB05 * 2 x Jack: NOB2,NOB4,NOB6
Testing (2026-6-25)
At 60 min (ONE HOUR) after training, repeat test with 2 different toys:
- wipe down sides of arena with Windex multisurface disinfectant cleaner
- place new wrap under arena
- place 1 kong and 1 jack in corners 20cm away from each wall
- bring rat cage from housing rm BRF169 (don't weigh rat again)
- start video recording on iMac, place rat in arena for 3 (THREE) minutes
- return to home cage and BRF 169.
- repeat for each rat, alternating position of jack and kong.
| AID | Left | Right |
|---|---|---|
| NB01 | kong* | jack |
| NB02 | kong | jack* |
| NB03 | jack | kong* |
| NB04 | jack* | kong |
| NB05 | kong* | jack |
| NB06 | jack* | kong |
* indicates familiar object
24-hour Retention Test
Habituation phase (2026-7-27,28,29)
- All 6 rats cages brought on cart from housing rm BRF169 to test room BRF115, weighed, placed in arena for 3 minutes, returned to home cage.
Training (2026-7-30)
At 24 hours after training:
- All 6 rats cages brought on cart from housing rm BRF169 to test room BRF115.
- place 2 identical toys (i.e., 2 x shroom or 2 x ball) in corners 20cm away from each wall
- rat weighed, placed in arena for 3 minutes.
* 2 x shroom: NB01, 03, 05 * 2 x ball: NB02,04,06
Testing (2026-7-32)
- All 6 rats cages brought on cart from housing rm BRF169 to test room BRF115.
- place 1 of each toys (shroom and ball) in corners 20cm away from each wall
- rat weighed, placed in arena for 3 minutes.
| AID | Left | Right |
|---|---|---|
| NB01 | shroom* | ball |
| NB02 | ball* | shroom |
| NB03 | shroom* | ball |
| NB04 | ball* | shroom |
| NB05 | shroom* | ball |
| NB06 | ball* | shroom |
* indicates familiar object
Analysis
Yolo Detection (2026-7-9)
yolo-detect.py run on NOB Training and Test movies, using pose model from 2026-7-6.