Methylation-Sensitive Enzymes
Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.
| Enzyme | NEB link | Site | Cut Position | Reaction Conditions |
|---|---|---|---|---|
| HpaII | https://www.neb.com/products/r0171-hpaii | CCgg | 5’...C/CGG...3' 3’...GGC/C...5’ |
1X NEBuffer 1, Incubate at 37°C. |
| MspI | https://www.neb.com/products/r0106-mspi | CCgg | 5’...C/CGG...3' 3’...GGC/C...5’ |
1X NEBuffer 4, Incubate at 37°C. |
| AciI | https://www.neb.com/products/r0551-aciip | CCgC | 5’...C/CGC...3' 3’...GGC/G...5’ |
1X NEBuffer 3, Incubate at 37°C. |
| HhaI | https://www.neb.com/products/r0139-hhai | gCgC | 5’...GCG/C...3' 3’...C/GCG...5’ |
1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C. |