| Valproate 100 mg/kg enhanced CTA | |
|---|---|
| HDAC Sex Differences | |
| Expt Code | DMQ |
| Start Date | 2021-12-14 |
| Investigators | Mukherjee, Houpt |
| Funding | Chair's Fund |
| Protocol | 1917 |
| Expt Type | CTA |
| Species | rat |
| Drugs | valproate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMQ |
Data
People
Durba Mukherjee
Agenda
Rats arrive (?????)
Sprague Dawley rats (dob ????? ), arrived
Day 0: Water removed 2021-12-20
Days 1-7: Water Restriction 2021-12-21 thru 2021-12-27
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
Day 8: Conditioning Day 2021-12-28
- -15 min: 8 ml/kg (100 mg/kg) of Valproate 75mm
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg ip of 0.15M LiCl or NaCl
Groups:
- DMQ01-04 Val-Li-Male
- DMQ05-08 Val-NaCl-Male
- DMQ09-12 Val-Li-Female
- DMQ13-16 Val-NaCl-Female
ad lib water returned after final injection overnight.
Days 9 - 21: Preference Tests: 2021-12-29 to 2022-1-10
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 12 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.