JF Systemic Sodium Selenate

JF Rats: Test Selenate Attenuation of Conditioned Taste Aversion (25 days)

Mon 2013-2-4

Rats (n= 12) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min).

Tue 2013-2-12

Weigh rats

Wed 2013-2-13 Conditioning day

• 4 groups (n=6 per group) NaCl/NaCl, Na2SeO4/NaCl, NaCl/LiCl, Na2SeO4/LiCl
• at - 2 h, inject with Na2SeO4 (0.5 mg/kg i.p.) or NaCl
• at 0 h, 10 min access to 0.125% saccharin
• at 0.5 h, inject with LiCl or NaCl (0.15 M, 6 ml/kg, i.p.)
• at 1.5 h, return water overnight

Thu 2013-2-14

Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days.

Thu 2013-2-28

Weigh bottles off and end experiment.

Polycose retest?

were JF rats retested with polycose paired with LiCl to determine if selenate had persistant effect on CTA learning?