MBJ Selenate Mass Spec
Background
Sodium Selenate (Na2Se04) activates PP2A (1).
CTA and Selenate experiments show that male Sprague Dawley rats given systemic injections of 0.5mg/kg sodium selenate two hours before taste aversion conditioning form attenuated taste aversions that extinguish rapidly. Selenate also decreases LiCl-induced phospho-MAP kinase in the NTS and lateral PBN.
This experiment will use LCMS to identify differences in protein phosphorylation in the brain two hours after rats receive 0.5mg/kg selenate vs vehicle.
Agenda
07/15/2023: 10 rats (5 males, 5 females) were overdosed with euthasol and rapidly decapitated two hours after receiving 1ml/kg i.p. injections of 0.5mg/kg sodium selenate or vehicle (0.15M NaCl). Brains were dissected and washed in ice-cold PP2A storage buffer (Tris-HCl, pH 7.4, EDTA, EGTA, DTT, Sucrose, N-PER, protease inhibitor tablet [will update to include concentrations]), and frozen at -80*C.
| Subject | Sex | Treatment | Weight | Injection vol (ml) | Injection time (PM) | Time of brain dissection (PM) |
|---|---|---|---|---|---|---|
| MBJO1 | M | NaCl | 371 | 0.37 | 2:17 | 4:28 |
| MBJ02 | F | Sel | 257 | 0.26 | 2:26 | 4:34 |
| MBJ03 | M | Sel | 348 | 0.35 | 2:33 | |
| MBJ04 | F | NaCl | 250 | 0.25 | 2:40 | |
| MBJ05 | M | NaCl | 346 | 0.35 | 2:46 | 5:00 |
| MBJ06 | F | Sel | 247 | 0.25 | 2:53 | |
| MBJ07 | M | Sel | 324 | 0.33 | 2:59 | |
| MBJ08 | F | NaCl | 245 | 0.25 | 3:04 | |
| MBJ09 | M | NaCl | 335 | 0.34 | 3:10 | |
| MBJ10 | F | Sel | 263 | 0.26 | 3:16 | 5:33 |