JCG Inhibitory DREADD Functional Validation

Agenda

Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)

Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.

Pre Treatment (1/28/24) 2 weeks before starting water restriction 4 groups:

1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed

2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed

Day 0: Water removed (**2024-7-19)

Days 1 - 11: Water Restriction (2023-9-13 - 2023-9-23)

Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.

Mice given water bottle and empty bottle, alternating position.

In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.

Day 12: Conditioning Day (2024-2-28)

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 15 min

Groups:

LTC: Exp group (Predicted to form taste aversion)
VVC: Control (expect to not form taste aversion)

JCG01-05: ???
JCG06-10: ???

After injection, ad lib water returned overnight.

Day 13 - 24: Preference Tests (2023-9-25 - 2023-10-6 )

CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.