Fluorescent Microscopy
Here are the immunofluorescence filters that we probably need. Bolded are the fluorophores we frequently use.
| Probe | Excitation (nm) | Emission (nm) | Color | |
| Neurotrace | 435 | 455 | blue | |
| DAPI | 358 | 461 | blue (often use mixed in VectaShield) | |
| DyLight 405 | 400 | 421 | blue | |
| Brilliant Violet 421 | 407 | 421 | blue (expensive) | |
| GFP | 425 | 500 | green | |
| Alexa Fluor 488 | 493 | 519 | green | |
| Fluorescein (FITC) | 492 | 520 | green | |
| YFP | 500 | 535 | yellow | |
| Cyanine Cy3 | 550 | 570 | orange | |
| Alexa Fluor 568 | 577 | 602 | red | |
| Texas Red | 589 | 615 | red | |
| Alexa Fluor 594 | 591 | 614 | red (sub for Texas Red) | |
| Cyanine Cy5 | 650 | 670 | red | |
| Alexa Fluor 647 | red, not for mercury lamps |
VECTASHIELD® PLUS Antifade Mounting Medium (H-1900)
VECTASHIELD® PLUS Antifade Mounting Medium with DAPI (H-2000)