Fluorescent Microscopy

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Here are the immunofluorescence filters that we probably need. Bolded are the fluorophores we frequently use.

Probe Excitation (nm) Emission (nm) Color
Neurotrace 435 455 blue
DAPI 358 461 blue (often use mixed in VectaShield)
DyLight 405 400 421 blue
Brilliant Violet 421 407 421 blue (expensive)
GFP 425 500 green
Alexa Fluor 488 493 519 green
Fluorescein (FITC) 492 520 green
YFP 500 535 yellow
Cyanine Cy3 550 570 orange
Alexa Fluor 568 577 602 red
Texas Red 589 615 red
Alexa Fluor 594 591 614 red (sub for Texas Red)
Cyanine Cy5 650 670 red
Alexa Fluor 647 red, not for mercury lamps

VECTASHIELD® PLUS Antifade Mounting Medium (H-1900)

VECTASHIELD® PLUS Antifade Mounting Medium with DAPI (H-2000)