RT-PCR Conditions for a bunch of genes implicated in Bipolar Disorder

DAOA (D-amino acid oxidase activator, aka G72)

DISC1 (disrupted in schizophrenia 1)

NRG1 (neuregulin1)

DTNBP1 (dystrobrevin binding protein 1)

PALB2 (partner and localizer of BRCA2)

NDUFAB1 (NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1)

DCTN5 (dynactin 5)

DAAO

Source: Konno 1998 PMID 12734589

Sense primer (F: 5’-GGTTAACTGAGAGGGGAGTGAA-3’)

Antisense primer (R: 5’- CCATAGTTGTGGATGACCTCTG-3’)

designed from the sequence conserved in cDNAs encoding human, mouse, rabbit, and pig D-amino-acid oxidases.

initial denaturation at 94°C for 1 min, 30 cycles of PCR

denaturation: 94°C, 30 sec

annealing: 55°C, 30 sec

extension: 72°C, 45 sec)

final extension was done at 72°C for 5 min

Serine Racemase

Source: Korostishevsky 2004 PMID 15271585

primers. For G72 gene forward: 5'-GCTGAATGAGAAGCA-3'; reverse: 5'-TGGGTCCCAGACACAGAGT-3';