DMZ Orchiectomy and Butyrate 3
Third cohort to test of role of testicular hormones in butyrate-enhanced CTA, follow-up to DMX Orchiectomy and Butyrate-Enhanced CTA and DMY Orchiectomy and Butyrate 2.
Mukherjee showed that HDAC inhibitors butyrate and calproate enchanced CTA in male but not female rats. Ovariectomy did not make female rats sensitive, so testing if orchiectomy reduces sensitivity of male rats.
| Orchiectomy and Butyrate 3 | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMZ |
| Start Date | 2025-6-15 |
| Investigators | Mukeherjee, Bass, Houpt |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | butyrate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMZ |
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID 20346924 PMCID: PMC2871962
- 0.15 M LiCl = 3.18g / 500ml water
- 0.3 M Na Butyrate = 3.3g/100ml water (Sigma 303410-100g, pcode 102622007, source BCCJ6385)
- 0.3 M NaCl = 1.8g/100ml water (osmotic control)
Data
People
Mukherjee, Bass, Houpt
Agenda
Rats arrive Male Sprague Dawley rats n = 16, d.o.b. 2025-3-27, arrived: 2025-5-27
Surgery
- DMZ 1-8: Orchiectomy Surgery on 2025-6-15
- DMZ 9-16: Sham Surgery on 2025-6-19
Orchiectomy as per Sophocles and Idris 2019 PMID 30729469
Sham rats received incision and staples.
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
Day 0: Water removed 2025-6-29
Days 1-6: Water Restriction 2025-7-5 thru 2025-7-12
note: this would be sooner than last 2 experiments -- consider pushing back a week
Weigh rats daily. Ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1400 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
Day 7: Conditioning Day 2025-7-13
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
- at 40 min: 12 ml/kg of 0.3M NaButyrate or 0.3M NaCl
ad lib water returned after final injection overnight.
Groups:
- ORC + LiCl + NaCl: DMZ01-04
- ORC + LiCl + Butyrate: DMZ05-08
- Sham + LiCl + NaCl: DMZ09-12
- Sham + LiCl + Butyrate: DMY13-16
Days 8 - 15: Preference Tests: 2025-7-14 to 2025-7-24
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 10 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.